Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 160,737 G→A 43.8% P343P (CCG→CCA degP → serine endoprotease (protease Do), membrane‑associated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE160,7370GA43.8% 19.3 / 55.2 73P343P (CCG→CCAdegPserine endoprotease (protease Do), membrane‑associated
Reads supporting (aligned to +/- strand):  ref base G (16/25);  new base A (14/18);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCCTCCGCTGCAAAAGCGGGCATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGA  >  minE/160673‑160798
                                                                |                                                             
aTTCCTCCGCTGCAAAAGCGGGCATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCGATc                                                            >  1:2027473/1‑68 (MQ=255)
aTTCCTCCGCTGCAAAAGCGGGCATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCGATc                                                            >  1:2049158/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:1341916/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2461343/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2463142/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2465246/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:240555/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2286303/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2867407/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:870078/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:873731/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:944677/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:1628162/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGAACTGCGTg                                      >  1:2632244/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACCTAACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:2965012/1‑68 (MQ=255)
                      cATTAAAGCGGGTGATGTGATCACATCACTGAACGGTAAGCCAATCAGCAGCTTTGCCGCACTGCGTg                                      >  1:654230/1‑68 (MQ=255)
                                    atgtgatCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTa                       >  1:776195/1‑69 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:521979/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:751602/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1055967/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:2679087/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:2733469/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:349996/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:527343/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1309794/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1904244/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1327741/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:137756/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1441039/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTa               >  1:1601599/1‑67 (MQ=255)
                                              cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAgg             <  1:1596561/69‑1 (MQ=255)
                                                 aCTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCa                                  <  1:3291157/45‑1 (MQ=255)
                                                 aCTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCa                                  <  1:3278874/45‑1 (MQ=255)
                                                 aCTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCa                                  <  1:2337355/45‑1 (MQ=255)
                                                 aCTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCa                                  <  1:1929923/45‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:2394152/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:3283689/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:768767/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:651479/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:3121361/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:2996441/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:2968564/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:2109685/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:810878/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:716099/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:19029/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:1194154/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:945901/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:1576689/69‑1 (MQ=255)
                                                   tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGgcagca        <  1:1872981/69‑1 (MQ=255)
                                                         gTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGa  <  1:433133/69‑1 (MQ=255)
                                                         gTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGa  <  1:3350702/69‑1 (MQ=255)
                                                         gTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGa  <  1:1965202/69‑1 (MQ=255)
                                                         gTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGa  <  1:1533438/69‑1 (MQ=255)
                                                         gTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGCAGGCAGCAAACTGa  <  1:3200077/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCTGTAGGTAGCAAACTGa  <  1:1385164/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTATCAAACTGa  <  1:736255/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTATCAAACTGa  <  1:259112/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTATCAAACTGa  <  1:1098724/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:476085/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:368390/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:568867/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:3192383/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:2987693/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:2815599/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:2615035/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:1974306/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:1970038/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:1696087/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:1351298/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:133879/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:126320/69‑1 (MQ=255)
                                                         gTAAGCCAATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGCACTATGCCGGTAGGTAGCAAACTGa  <  1:1077257/69‑1 (MQ=255)
                                                                |                                                             
ATTCCTCCGCTGCAAAAGCGGGCATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGA  >  minE/160673‑160798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: