| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,988,048 | A→G | 100% | N190N (AAT→AAC) | yggN ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,988,048 | 0 | A | G | 75.1% | 56.4 / 14.0 | 48 | N190N (AAT→AAC) | yggN | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/8); new base G (36/0); total (40/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT > minE/1987983‑1988112 | tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTc > 1:485668/1‑69 (MQ=255)tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTc > 1:1171556/1‑69 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTc > 1:3307528/1‑68 (MQ=255) tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:590867/66‑1 (MQ=255) tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:1550748/66‑1 (MQ=255) tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:3096975/66‑1 (MQ=255) tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:2921472/66‑1 (MQ=255) tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCACCAGGCTTCCCAGCACATTCTGt < 1:1497147/66‑1 (MQ=255) ccTGCTTTTTCCACTCGGTTTGGTTTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:2887876/65‑1 (MQ=255) tGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGc > 1:1357932/1‑69 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa > 1:1440397/4‑67 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa > 1:2284693/4‑67 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1053477/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2933317/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:547857/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2724675/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2672707/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2236931/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2150003/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2137462/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2070887/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1099170/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:179672/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1705243/4‑68 (MQ=255) ccgcccAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1168623/4‑68 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCt > 1:487577/1‑39 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCttt > 1:1294807/1‑54 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa > 1:656089/1‑62 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa > 1:3299737/1‑62 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:980782/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2860311/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2852368/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2538539/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1623795/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:1980335/1‑63 (MQ=255) cAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:2098865/1‑63 (MQ=255) cAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt < 1:974703/69‑1 (MQ=255) cAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt < 1:3055233/69‑1 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:1990767/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:2607244/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:1387003/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:1289433/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:29943/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:2288572/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:1162253/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:1022939/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCGTTCGCGCCCATTTCATTAAt > 1:1853832/1‑69 (MQ=255) gCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCGTTTCAGCACCGCTTTCGCGCCCATTTCAtt > 1:2883542/1‑66 (MQ=255) cacaTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:346584/1‑60 (MQ=255) cacaTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt > 1:1867425/1‑68 (MQ=255) cacaTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt > 1:1138710/1‑68 (MQ=255) cacaTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt > 1:396581/1‑68 (MQ=255) cacaTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt > 1:1643260/1‑68 (MQ=255) | TCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT > minE/1987983‑1988112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |