Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,990,804	C→T	60.0%	D266D (GAC→GAT)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,990,804	0	C	T	60.0%	10.8 / 21.6	35	D266D (GAC→GAT)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base T (12/9);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCG  >  minE/1990739‑1990850                                                                  |                                               ttGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCcgacga                                              >  1:422896/1‑68 (MQ=255)   gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa                                             >  1:249854/1‑67 (MQ=255)   gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa                                             >  1:1826213/1‑67 (MQ=255)   gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa                                             >  1:1709592/1‑67 (MQ=255)   gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa                                             >  1:1292614/1‑67 (MQ=255)                          gATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGgcgc                    <  1:2895396/69‑1 (MQ=255)                          gATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGgcgc                    <  1:1190973/69‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:1238208/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:1140228/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:86625/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:591422/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:2709487/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:2705676/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:2370420/56‑1 (MQ=255)                           aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:2696811/56‑1 (MQ=255)                             tgtgtgGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc                                <  1:2497224/54‑1 (MQ=255)                             tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGc                                >  1:1416088/1‑54 (MQ=255)                                 gggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa             <  1:1001024/69‑1 (MQ=255)                                 gggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa             <  1:2345795/69‑1 (MQ=255)                                   gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg            <  1:2421730/68‑1 (MQ=255)                                   gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg            <  1:2361877/68‑1 (MQ=255)                                   gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg            <  1:2978657/68‑1 (MQ=255)                                    cGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg            <  1:1284837/67‑1 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                 >  1:572377/1‑57 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                 >  1:1038267/1‑57 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                 >  1:2579327/1‑57 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                 >  1:1060487/1‑57 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:3065777/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:3136163/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:3263653/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:3379563/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:2898857/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:559821/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:215937/1‑69 (MQ=255)                                         tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg     >  1:957458/1‑69 (MQ=255)                                             cTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCg  >  1:733463/1‑68 (MQ=255)                                                                  |                                               TTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCG  >  minE/1990739‑1990850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑