| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,407 | C→T | 49.5% | S357S (AGC→AGT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,407 | 0 | C | T | 49.5% | 0.5 / 102.3 | 111 | S357S (AGC→AGT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (48/8); new base T (37/18); total (85/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTA > minE/1346‑1472 | cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:60138/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:1734047/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:3258971/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:3283859/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:2810586/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:421213/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:2103110/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:1482975/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:2950126/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:126215/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:812385/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:86690/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:2212827/68‑1 (MQ=255)cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:1996492/68‑1 (MQ=255) cGTATTTCCGTGGTGCTGATTACTCAATCATCTTCCGAATACAGTATCAGt < 1:1366640/51‑1 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGa > 1:671280/1‑69 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGa > 1:1923881/1‑69 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCg > 1:2674659/1‑54 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCg > 1:1645818/1‑54 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:2993973/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:2942608/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:3047018/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:3115310/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:3132401/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:1732853/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:2844779/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:3399534/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:591939/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:609302/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:278935/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:74706/1‑55 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGt > 1:25814/1‑55 (MQ=255) tCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACtgt > 1:239848/1‑69 (MQ=255) tCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACtgt > 1:346872/1‑69 (MQ=255) tgCTGATTACGCAATCATCTTCCGAATACAGTATCAGt < 1:1232243/38‑1 (MQ=255) gCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAg > 1:1496921/1‑68 (MQ=255) gCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAg > 1:1320589/1‑68 (MQ=255) gCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAg > 1:2702556/1‑68 (MQ=255) gCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAg > 1:1071556/1‑68 (MQ=255) gCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAg > 1:2272021/1‑68 (MQ=255) gATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTg < 1:2628334/68‑1 (MQ=255) gATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTg < 1:1053611/68‑1 (MQ=255) gATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGa > 1:1963657/1‑69 (MQ=255) gATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGa > 1:3369146/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTgca > 1:2654868/1‑66 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:932312/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:737326/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:640932/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:1368094/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:759697/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:3412293/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:1666573/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:1680694/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:777314/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:3167790/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:89657/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:2993233/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:931418/1‑69 (MQ=255) ttACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:2007405/1‑69 (MQ=255) ttACGCAATCATCTTCAGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:2568944/1‑69 (MQ=255) ttACCCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:2332355/1‑69 (MQ=255) ttACACAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:539096/1‑69 (MQ=255) tACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACg > 1:2154878/1‑69 (MQ=255) tACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACg > 1:2408588/1‑69 (MQ=255) cAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc < 1:633931/68‑1 (MQ=255) cAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc < 1:1838629/68‑1 (MQ=255) cAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc < 1:1825149/68‑1 (MQ=255) aaTCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc < 1:1587322/67‑1 (MQ=255) catcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATg > 1:1871261/1‑68 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1019633/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1967622/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:2245402/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:368251/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1231635/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1237319/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:2199652/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:2704927/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:3211618/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:210972/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1196837/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:2953778/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:1892975/1‑63 (MQ=255) atcTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:2897515/1‑63 (MQ=255) cTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 1:347217/1‑67 (MQ=255) cTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 1:1758602/1‑67 (MQ=255) cTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 1:1441740/1‑67 (MQ=255) cTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 1:1833879/1‑67 (MQ=255) tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 1:1710295/1‑67 (MQ=255) tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 1:696452/1‑67 (MQ=255) tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 1:1630216/1‑67 (MQ=255) tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 1:313620/1‑67 (MQ=255) tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 1:2539982/1‑67 (MQ=255) tgcAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc > 1:3271807/3‑69 (MQ=255) tACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc > 1:883666/1‑69 (MQ=255) tACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc > 1:3008634/1‑69 (MQ=255) cAGCATCAGTTTCTGCGTTCCACAAAGCGACtgtgt > 1:1387294/1‑36 (MQ=255) cAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTa > 1:1129480/1‑69 (MQ=255) cAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTa > 1:883248/1‑69 (MQ=255) cAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTa < 1:896697/69‑1 (MQ=255) cAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTa < 1:2003272/69‑1 (MQ=255) | CAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTA > minE/1346‑1472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |