| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 22,613 | T→G | 33.3% | S849A (TCG→GCG) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 22,613 | 0 | T | G | 33.3% | 94.2 / 85.9 | 108 | S849A (TCG→GCG) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (66/6); new base G (15/21); total (81/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCT > minE/22556‑22672 | aaGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTg < 1:635094/69‑1 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:440342/1‑69 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:1039923/1‑69 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:1152770/1‑69 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:341247/1‑69 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:1269348/1‑69 (MQ=255) aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGAcc > 1:3001427/1‑69 (MQ=255) tgggtggCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCgcgc > 1:1705449/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGCACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:1599379/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTAc > 1:1994916/1‑68 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:151712/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:540152/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:210808/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:1562045/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:691277/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:2936507/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:2297807/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:1302842/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:841612/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:1200832/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:2331589/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:2709405/1‑69 (MQ=255) aGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg > 1:2515635/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGCAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCt > 1:3059904/1‑68 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1771790/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:2174367/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:2119709/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:3239446/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:3298183/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1918827/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:852805/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:7242/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1678553/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:649514/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1596986/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1439438/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcc > 1:1372241/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTc > 1:1185859/1‑66 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCt > 1:2367526/1‑68 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:858297/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:2846490/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:2931388/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:65781/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:3177762/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:61571/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:1029095/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:3339241/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:590699/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:513913/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:1660902/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:202595/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTg > 1:2165313/1‑69 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACAGCGCTGGGCGATGAATTACGATTTGTcc > 1:3383705/1‑67 (MQ=255) aCCTTGTATGCAGAACCGGAACTGTCGCCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCt > 1:3416132/1‑68 (MQ=255) cTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt < 1:1234732/69‑1 (MQ=255) cTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt < 1:2041892/69‑1 (MQ=255) cTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt < 1:3299978/69‑1 (MQ=255) cTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt < 1:2086920/69‑1 (MQ=255) cTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt < 1:3135461/69‑1 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGAcc > 1:3043956/1‑67 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTc > 1:1704980/1‑69 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTc > 1:404797/1‑69 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTc > 1:3416842/1‑69 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTc > 1:1646345/1‑69 (MQ=255) tGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTc > 1:789693/1‑69 (MQ=255) cAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTcc > 1:2770379/1‑68 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTTGGc < 1:3255439/36‑1 (MQ=25) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2268909/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:254642/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:1868943/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:188355/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2725697/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:353645/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:274265/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2026528/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2829088/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:3345169/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2290835/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2045921/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2134265/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2278689/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:1515417/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:3077759/36‑1 (MQ=255) gAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGc < 1:2174483/36‑1 (MQ=255) ccGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGgcgc > 1:1334140/1‑68 (MQ=255) ccGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCt > 1:2838639/1‑69 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:824689/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:1030759/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:1666014/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:1024934/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:1802782/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:544783/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:3367433/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:3119261/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:3110069/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:2898792/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:2767216/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:2491555/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:2615207/1‑48 (MQ=255) gTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGtt > 1:2585152/1‑48 (MQ=255) | AAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCT > minE/22556‑22672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |