| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 160,821 | T→G | 47.1% | G371G (GGT→GGG) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 160,821 | 0 | T | G | 47.1% | ‑4.1 / 58.6 | 51 | G371G (GGT→GGG) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base T (12/15); new base G (9/15); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
CGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCA > minE/160759‑160885 | cGTGCTCAGGTGGGTACTATGCCGGTAGGCATCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:819676/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:2969436/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:1907037/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:2528821/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:2080389/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:2323592/1‑69 (MQ=255)cGTGCTCAGGTGGGTACTATGCCGCTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAg > 1:552575/1‑69 (MQ=255) cTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTa > 1:1307332/1‑69 (MQ=255) gggTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGtt < 1:2237105/61‑1 (MQ=255) gggTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGtt < 1:79829/61‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCTGGTTAACGTGAAc < 1:2165978/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2585408/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2247314/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2256738/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:110636/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2400414/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:3001569/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:523180/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:877457/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2122138/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:1263161/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:1156406/66‑1 (MQ=255) ggcaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:2232952/66‑1 (MQ=255) ggaaCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:1236610/66‑1 (MQ=255) aCTATGCCGGTAGGTAGCAAACTGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAAc < 1:3005434/66‑1 (MQ=255) cTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTgg < 1:2373888/69‑1 (MQ=255) cTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTgg < 1:2410593/69‑1 (MQ=255) cTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTgg < 1:2597155/69‑1 (MQ=255) ggCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTgg < 1:574136/58‑1 (MQ=255) cTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGTACCTGGAACTGCAGCAGAGCAGCCAg > 1:589471/1‑69 (MQ=255) cTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAg > 1:252894/1‑69 (MQ=255) cTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAg > 1:1723437/1‑69 (MQ=255) cTGACCCTGGGCTTACTGCGCGACGGTAAGCAGCTTAACGTGAACCTGGAACTTCagcagagcag > 1:2644488/1‑65 (MQ=255) tgccccTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCTGCAGAGCAGCCAg > 1:3275433/4‑68 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:1757585/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:1721460/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:735316/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:2330805/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:736371/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:2526826/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:2410233/1‑69 (MQ=255) tGACCCTGGGCTTACTGCGCGACGGGAAGCAGGTTAACGTGAACCTGGAACTTCAGCAGAGCAGCCAGa > 1:1966981/1‑69 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:1107279/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:1299970/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:45446/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:345075/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:1922771/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:23800/67‑1 (MQ=255) ccTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAAt < 1:2017415/67‑1 (MQ=255) tGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTc < 1:2685624/67‑1 (MQ=255) aCGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCa < 1:2501991/69‑1 (MQ=255) | CGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCA > minE/160759‑160885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |