| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,996,903 | G→A | 23.1% | G119G (GGG→GGA) | yqgA → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,996,903 | 0 | G | A | 23.1% | 53.5 / 10.3 | 43 | G119G (GGG→GGA) | yqgA | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (22/11); new base A (5/5); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
AATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTT > minE/1996845‑1996969 | aaTCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCggg > 1:1658422/1‑68 (MQ=255)aaTCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCggg > 1:842177/1‑68 (MQ=255) aaTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg < 1:996867/68‑1 (MQ=255) aaTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg < 1:3155885/68‑1 (MQ=255) aaTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg < 1:243858/68‑1 (MQ=255) aaTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg < 1:83313/68‑1 (MQ=255) tATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATg < 1:1954862/69‑1 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTc > 1:863328/1‑47 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACgca > 1:3232197/1‑60 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACTAAGGGATGa > 1:3084534/1‑69 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGa > 1:1407017/1‑61 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGa > 1:2845020/1‑61 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATg > 1:2554072/1‑68 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:2141963/1‑69 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:1187003/1‑69 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:3366417/1‑69 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:2622457/1‑69 (MQ=255) aTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:1740062/1‑69 (MQ=255) tattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTc > 1:125179/1‑37 (MQ=255) tattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg > 1:2624091/1‑54 (MQ=255) tattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg > 1:29092/1‑54 (MQ=255) tattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg > 1:3249944/1‑54 (MQ=255) gTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGt < 1:2308055/69‑1 (MQ=255) gTTTTGCGCCAGCGGCACCGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTAtttt > 1:691998/1‑69 (MQ=255) gTTTTGCGCCAGCGGCACCGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTAtttt > 1:2688038/1‑69 (MQ=255) gTTTTGCGCCAGCGGCACCGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTAtttt > 1:2737996/1‑69 (MQ=255) gTTTTGCGCCAGCGGCACCGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTAtttt > 1:1738392/1‑69 (MQ=255) gTTTTGCGCCAGCGGCACCGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTAtttt > 1:3149645/1‑69 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAgg < 1:312370/42‑1 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGa > 1:726724/1‑47 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTa < 1:139190/67‑1 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTa < 1:3041551/67‑1 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTa < 1:3178936/67‑1 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAAt > 1:2642780/1‑69 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAAt > 1:2575028/1‑69 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAAt > 1:2412978/1‑69 (MQ=255) ttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAAt > 1:1549245/1‑69 (MQ=255) cAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTa < 1:2535285/61‑1 (MQ=255) ccGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc < 1:2961195/69‑1 (MQ=255) ccGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc < 1:2816883/69‑1 (MQ=255) ccGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc < 1:1003556/69‑1 (MQ=255) ccGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc < 1:1408267/69‑1 (MQ=255) ccGGAATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc < 1:1111646/69‑1 (MQ=255) gggATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCtt > 1:135393/1‑69 (MQ=255) | AATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTT > minE/1996845‑1996969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |