| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,000,404 | G→A | 43.6% | I293I (ATC→ATT) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,000,404 | 0 | G | A | 43.6% | 37.3 / 92.0 | 119 | I293I (ATC→ATT) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base G (31/36); new base A (23/29); total (54/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCA > minE/2000336‑2000468 | cGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCggg > 1:964590/1‑69 (MQ=255)cGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCggg > 1:775082/1‑69 (MQ=255) aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGaa > 1:1211389/1‑68 (MQ=255) aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:2304579/1‑69 (MQ=255) aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:3114384/1‑69 (MQ=255) aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:3155430/1‑69 (MQ=255) aTCATATGGTGACAGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:1318246/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1012029/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2062611/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:216071/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2245340/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2957400/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2983156/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1603455/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1577229/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:3265999/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:620158/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1147329/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:890443/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1176139/1‑69 (MQ=255) aTCATATGATGACGGCGACGCTGCAAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:117827/1‑69 (MQ=255) aTCATAGGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:440321/1‑69 (MQ=255) tatatGGTGACGGAGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg > 1:1912942/2‑69 (MQ=255) cATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg > 1:1673389/1‑69 (MQ=255) cATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg > 1:243935/1‑69 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2026723/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2028223/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2244507/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:178561/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1697797/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1221748/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:718485/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1546710/1‑67 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:2072022/1‑69 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:1113745/1‑69 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:3230435/1‑69 (MQ=255) gTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:713278/1‑67 (MQ=255) gACGGCGACGCTGCCAGGAGAGACGCAAACGTGG‑CAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:2433765/69‑1 (MQ=255) actgCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1683854/66‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:914342/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:1223797/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2024159/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:127603/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2143290/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:3167279/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1236949/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1719247/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2411637/69‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1573374/68‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:3178709/68‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2759977/68‑1 (MQ=255) cGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:616133/62‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:1403541/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:2677640/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:173629/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:1213053/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:73181/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:16570/69‑1 (MQ=255) tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt < 1:741178/69‑1 (MQ=255) tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt < 1:3000952/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2448416/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:673733/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:402766/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:385770/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:368401/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:931025/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:1718118/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2930415/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:232441/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2713314/69‑1 (MQ=255) gCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:798580/68‑1 (MQ=255) ggCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:648232/60‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:517653/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:711995/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:377723/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:2472643/69‑1 (MQ=255) agGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa < 1:1884461/69‑1 (MQ=255) agGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa < 1:3290138/69‑1 (MQ=255) ggATTTTCGGGATGTCGAACAGTGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa > 1:2844439/1‑68 (MQ=255) ggATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa > 1:1880038/1‑68 (MQ=255) ggATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa > 1:2149814/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:813206/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:2047401/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:1675583/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:1786702/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:2175525/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:2159196/1‑68 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:456162/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:69413/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:3219157/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:1706787/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:2788911/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:2071786/69‑1 (MQ=255) ttttCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:50546/68‑1 (MQ=255) ttttCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:3106407/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:468608/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:963303/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:1963391/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGGCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:1187539/68‑1 (MQ=255) gATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:1562094/61‑1 (MQ=255) | CGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCA > minE/2000336‑2000468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |