| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,000,410 | G→A | 36.3% | F291F (TTC→TTT) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,000,410 | 0 | G | A | 36.3% | 98.9 / 87.5 | 143 | F291F (TTC→TTT) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base G (54/37); new base A (23/29); total (77/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
ATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGC > minE/2000345‑2000476 | aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGaa > 1:1211389/1‑68 (MQ=255)aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:2304579/1‑69 (MQ=255)aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:3114384/1‑69 (MQ=255)aTCATATGGTGACAGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc > 1:1318246/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:3265999/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:216071/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2245340/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1012029/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:2957400/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1577229/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:3377322/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:620158/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1233847/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1147329/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:1176139/1‑69 (MQ=255)aTCATATGATGACGGCGACGCTGCAAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:117827/1‑69 (MQ=255)aTCATAGGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc > 1:440321/1‑69 (MQ=255) cATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg > 1:1673389/1‑69 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGTACAgc > 1:1488130/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2563764/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:178561/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1221748/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1697797/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2028223/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:1546710/1‑67 (MQ=255) atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc > 1:2200845/1‑67 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCTGAGGATTTTCGGGATGTCGAACAGCGcc > 1:2908686/1‑69 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:2350718/1‑69 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:1113745/1‑69 (MQ=255) tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:3230435/1‑69 (MQ=255) gTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc > 1:713278/1‑67 (MQ=255) gACGGCGACGCTGCCAGGAGAGACGCAAACGTGG‑CAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:2433765/69‑1 (MQ=255) actgCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1683854/66‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:914342/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag < 1:1223797/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:3167279/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2411637/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:911863/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1719247/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:127603/69‑1 (MQ=255) aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2024159/69‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:2759977/68‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:3178709/68‑1 (MQ=255) cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:1573374/68‑1 (MQ=255) cGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag < 1:616133/62‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:606086/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:737645/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:73181/69‑1 (MQ=255) cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc < 1:1618167/69‑1 (MQ=255) tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt < 1:3000952/69‑1 (MQ=255) tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt < 1:741178/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:3024725/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:402766/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:1718118/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2930415/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:368401/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:931025/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2448416/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2043057/69‑1 (MQ=255) cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:2713314/69‑1 (MQ=255) ggCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg < 1:648232/60‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:711995/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:1760753/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:377723/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:2073934/69‑1 (MQ=255) agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa < 1:517653/69‑1 (MQ=255) agGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa < 1:1884461/69‑1 (MQ=255) ggATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa > 1:2149814/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:2159196/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:813206/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:1675583/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:2047401/1‑68 (MQ=255) ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa > 1:1786702/1‑68 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:456162/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:69413/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:2071786/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:1884516/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:2788911/69‑1 (MQ=255) aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:3219157/69‑1 (MQ=255) ttttCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc < 1:3106407/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:963303/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:1963391/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:468608/68‑1 (MQ=255) cGGGATGTCGAACAGCGCCAGCAGGTTGGCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa < 1:1187539/68‑1 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAgg > 1:3314308/1‑63 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGc > 1:2986861/1‑66 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:1343231/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2910978/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:675673/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:1412233/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2656574/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2450666/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:42619/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:1639902/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2241151/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2120032/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:825787/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:1238282/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:2065547/1‑69 (MQ=255) tCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGc > 1:1702877/1‑69 (MQ=255) | ATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCAGGTGCAGC > minE/2000345‑2000476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |