| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 160,882 | T→A | 24.1% | S392T (TCC→ACC) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 160,882 | 0 | T | A | 24.1% | 188.0 / 56.0 | 116 | S392T (TCC→ACC) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base T (42/46); new base A (21/7); total (63/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
ACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAAC > minE/160817‑160950 | aCGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCa < 1:2501991/69‑1 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:1036479/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:3429515/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc < 1:1726796/69‑1 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:374687/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:3407888/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:1776039/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:2514281/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:2583136/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:525720/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:799904/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:1382842/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:1300703/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:1273979/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:3249031/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc > 1:3121424/1‑69 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc < 1:2356259/69‑1 (MQ=255) aaGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTc < 1:213581/69‑1 (MQ=255) tAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACgg < 1:1323993/66‑1 (MQ=255) tAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCa > 1:1495623/1‑68 (MQ=255) tAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCa > 1:268341/1‑68 (MQ=255) cGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTg < 1:1351210/68‑1 (MQ=255) cGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTg < 1:1273237/68‑1 (MQ=255) cGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTg < 1:1224268/68‑1 (MQ=255) cGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTg < 1:1598804/68‑1 (MQ=255) gTGAACCTGGAACTGCATCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:2604728/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:1553795/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:355802/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:1602469/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:422543/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:1292418/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:2760972/68‑1 (MQ=255) gTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:2765122/68‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCTGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:854702/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2859896/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:292468/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:3290584/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2926489/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2702702/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2265567/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2200653/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:218877/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:3158380/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:904902/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:857387/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:4824/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:3294450/69‑1 (MQ=255) ggAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:1989808/69‑1 (MQ=255) ggAACTGCAGCAAAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTga < 1:2164716/69‑1 (MQ=255) aCTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTg < 1:200517/56‑1 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa > 1:2827686/1‑54 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:32493/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:1937849/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:2454325/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:2932878/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:702642/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:1209077/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:201015/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:903784/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:2335080/1‑68 (MQ=255) gcagcaGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGa > 1:3137850/1‑68 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:917214/69‑1 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:2851299/69‑1 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:2767419/69‑1 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:1456396/69‑1 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:1901028/69‑1 (MQ=255) cagagcagCCAGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGcaac < 1:2436764/69‑1 (MQ=255) gcagcCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 1:1044867/46‑1 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCATCGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1954852/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:3290966/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:588618/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:522312/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:514492/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1402638/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1706736/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1844989/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1054854/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1939565/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:322917/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:1160290/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggta > 1:3141918/1‑64 (MQ=255) aGAATCAGGTTGATTCCAGCACCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAggt > 1:173603/1‑64 (MQ=255) aTCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATc > 1:610273/1‑69 (MQ=255) ccAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGt > 1:2204008/1‑67 (MQ=255) ccAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTa > 1:2500109/1‑68 (MQ=255) ccAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTa > 1:2054457/1‑68 (MQ=255) ccAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTa > 1:1416576/1‑68 (MQ=255) ccAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTa > 1:1124251/1‑68 (MQ=255) gCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTg < 1:1555520/68‑1 (MQ=255) gCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTg < 1:2578669/68‑1 (MQ=255) tCCGTCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:3110737/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:1035110/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:1468435/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:1604731/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:1672086/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:3189449/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:2393834/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:3057858/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:2528418/1‑69 (MQ=255) tCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGaac > 1:2722977/1‑69 (MQ=255) | ACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAAC > minE/160817‑160950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |