| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,000,557 | A→G | 15.9% | G242G (GGT→GGC) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,000,557 | 0 | A | G | 15.9% | 86.7 / 7.8 | 56 | G242G (GGT→GGC) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (26/21); new base G (2/7); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
TCCTGCTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTG > minE/2000498‑2000622 | tCCTGCTCGGCACTCTCGGTAATGGTGTAGTAATGATACGGATCCTGATTTATCACCTGGCCGTTGGcc > 1:2062418/1‑69 (MQ=255)tCCTGCTCGGCACTCTCGGTAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGcc > 1:3044948/1‑69 (MQ=255) gCTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGca < 1:1080208/69‑1 (MQ=255) gCTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGca < 1:1246745/69‑1 (MQ=255) cTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGca > 1:2196367/1‑68 (MQ=255) cTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGca > 1:1179058/1‑68 (MQ=255) cTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGATGGCCTGc > 1:489217/1‑67 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:1038797/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:2546533/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:2852490/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:288834/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:312384/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTTGGCCTGTACATAGGCGtttt < 1:975532/66‑1 (MQ=255) tAATGGTGTAGTAATGATACGGATCCTGATTGATCACCTGGCCGTAGGCCTGTACATAGGCGtttt < 1:2862550/66‑1 (MQ=255) gtaATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCttt > 1:1825523/1‑69 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGca > 1:2677683/1‑41 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:1351246/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:906145/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:813748/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:1822045/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:1575217/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCg > 1:1282506/1‑49 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTc > 1:2852803/1‑69 (MQ=255) aTGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTc > 1:1446215/1‑69 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCtttttaa > 1:683500/1‑65 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:732081/1‑67 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:3001311/1‑67 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:2513511/1‑67 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:2509803/1‑67 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:1227029/1‑67 (MQ=255) aTAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCa > 1:1595469/1‑67 (MQ=255) agtaTCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:3396053/66‑1 (MQ=255) aGGATCGTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:363397/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:3154529/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:1075226/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:111915/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:3407300/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:2277631/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:3028231/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:225442/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:1881469/69‑1 (MQ=255) aGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAg < 1:2754017/69‑1 (MQ=255) gATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGcc < 1:1107491/69‑1 (MQ=255) cTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCAttt < 1:1687092/69‑1 (MQ=255) cTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCAttt < 1:810731/69‑1 (MQ=255) cTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCAttt < 1:1223149/69‑1 (MQ=255) tCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCa < 1:2199646/68‑1 (MQ=255) tCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCa < 1:2821665/68‑1 (MQ=255) tCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCa < 1:780320/68‑1 (MQ=255) tCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCa < 1:58950/68‑1 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACt > 1:134082/1‑68 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTg > 1:164765/1‑69 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTg > 1:2019688/1‑69 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTg > 1:3221998/1‑69 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTg > 1:1519579/1‑69 (MQ=255) cTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTg > 1:1526892/1‑69 (MQ=255) | TCCTGCTCGGCACTCTCGGTAATGGTGTAGTAATGATAAGGATCCTGATTGATCACCTGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTG > minE/2000498‑2000622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |