| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,001,010 | T→A | 16.2% | R91R (CGA→CGT) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,001,010 | 0 | T | A | 16.2% | 173.8 / 23.0 | 86 | R91R (CGA→CGT) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base T (49/23); new base A (7/7); total (56/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACAC > minE/2000954‑2001068 | cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTGTCGCAGGAAgcg > 1:2145754/1‑68 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:1066961/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:1794406/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:1813201/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:2075934/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:1524404/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:2315887/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:2533970/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:1264780/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:3272174/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:334637/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:772987/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:804088/1‑69 (MQ=255)cgcgGCGAGCCGTTAGGAAATGACTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAgcgc > 1:2254072/1‑69 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:2722276/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:2866742/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:2265844/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:989634/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:1666007/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:1469797/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTGACCACTTCACGCAgg < 1:121291/54‑1 (MQ=38) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:1549536/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:250950/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:2500718/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:241190/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:1206742/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:273266/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:2797728/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:2084541/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:2084401/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:1797533/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:935057/69‑1 (MQ=255) ccGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGaaa < 1:942400/69‑1 (MQ=255) gTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAAt < 1:364635/69‑1 (MQ=255) gTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAAt < 1:2430722/69‑1 (MQ=255) gTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAAt < 1:1182599/69‑1 (MQ=255) gAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTcc < 1:928914/68‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:2331084/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:44620/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:2587500/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:681924/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:1161286/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:1530490/69‑1 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:160150/69‑1 (MQ=255) gCAGGATGTTGTCATTTACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:3069447/1‑67 (MQ=255) gCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:3118337/1‑67 (MQ=255) gCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:312684/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:911761/1‑53 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:91481/1‑53 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:3191853/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1679859/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:350201/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:381694/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:608032/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:857385/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1028418/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1641074/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1554504/1‑67 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:2960428/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1346954/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:2563893/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1305184/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:282534/1‑66 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:670023/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:881024/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:2648724/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:361418/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:344468/1‑48 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCATGTCAGTGAAGACtaca > 1:2939684/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:571920/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:345760/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:639275/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:1632361/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:862634/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:3115607/4‑69 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAACTCTCCCACGc > 1:1447918/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:325304/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:964126/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:215726/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:2235055/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:818617/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1958547/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1772547/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1086433/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:3186526/1‑68 (MQ=255) cACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATAcc > 1:3257268/1‑45 (MQ=255) | CGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACAC > minE/2000954‑2001068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |