| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,001,040 | C→T | 25.8% | A81A (GCG→GCA) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,001,040 | 0 | C | T | 25.8% | 91.9 / 26.4 | 77 | A81A (GCG→GCA) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base C (39/18); new base T (7/13); total (46/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACG > minE/2000974‑2001106 | tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:2331084/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:44620/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:1161286/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:160150/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:681924/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:2587500/69‑1 (MQ=255)tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc < 1:1530490/69‑1 (MQ=255) gCAGGATGTTGTCATTTACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:3069447/1‑67 (MQ=255) gCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:312684/1‑67 (MQ=255) gCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCAc > 1:3118337/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:91481/1‑53 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:911761/1‑53 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:857385/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1028418/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:608032/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:3191853/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:350201/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:381694/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1679859/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1641074/1‑67 (MQ=255) aTGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1554504/1‑67 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1305184/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:1346954/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:2960428/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:282534/1‑66 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAg > 1:2563893/1‑66 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:881024/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:344468/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:2648724/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:670023/1‑48 (MQ=255) gtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:361418/1‑48 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCATGTCAGTGAAGACtaca > 1:2939684/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:1632361/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:862634/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:3115607/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:639275/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:571920/4‑69 (MQ=255) ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:345760/4‑69 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAACTCTCCCACGc > 1:1447918/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:2235055/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:325304/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:964126/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:818617/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGc > 1:215726/1‑42 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1958547/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1772547/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:3186526/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1086433/1‑68 (MQ=255) cACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATAcc > 1:3257268/1‑45 (MQ=255) cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:2064612/67‑1 (MQ=255) cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:927569/67‑1 (MQ=255) gAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAaga > 1:2506944/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:1721002/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:13784/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:2108029/1‑68 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:497315/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:687833/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:965294/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1044477/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1092644/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:30902/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1226896/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2542025/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2456589/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1278680/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1828098/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1779516/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACATCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:500366/59‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2159627/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1257385/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1471895/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2075809/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:365229/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2591575/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2972366/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3101310/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3204295/68‑1 (MQ=255) | TGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACG > minE/2000974‑2001106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |