| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,001,058 | A→G | 44.4% | F75F (TTT→TTC) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,001,058 | 0 | A | G | 44.4% | 4.5 / 34.3 | 45 | F75F (TTT→TTC) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base G (7/13); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
TTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAC > minE/2000999‑2001115 | ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCATGTCAGTGAAGACtaca > 1:2939684/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:862634/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:639275/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:571920/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:1632361/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:3115607/4‑69 (MQ=255)ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:345760/4‑69 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1086433/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1958547/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:3186526/1‑68 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1772547/1‑68 (MQ=255) cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:927569/67‑1 (MQ=255) cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:2064612/67‑1 (MQ=255) gAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAaga > 1:2506944/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:1721002/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:13784/1‑68 (MQ=255) aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:2108029/1‑68 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:497315/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:687833/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:30902/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1044477/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2542025/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:965294/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1779516/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1092644/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1226896/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1278680/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1828098/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2456589/59‑1 (MQ=255) gaAAATCTCCCATGCCATACCCACATCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:500366/59‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2075809/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2591575/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1257385/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1471895/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:365229/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3204295/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2159627/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3101310/68‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2972366/68‑1 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGATCAAATTCAACg > 1:2479479/1‑60 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCa > 1:2717520/1‑51 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCa > 1:1101270/1‑51 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg > 1:1683298/1‑60 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg > 1:6277/1‑60 (MQ=255) cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAc > 1:1796196/1‑69 (MQ=255) | TTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAC > minE/2000999‑2001115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |