| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,002,536 | A→G | 43.9% | D63D (GAT→GAC) | hybG ← | hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,002,536 | 0 | A | G | 43.9% | 21.8 / 78.0 | 98 | D63D (GAT→GAC) | hybG | hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (35/20); new base G (32/11); total (67/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATC > minE/2002485‑2002598 | ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:2431461/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:181134/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:1478761/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:1453362/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:2143653/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:215782/69‑1 (MQ=255)ggTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:2733878/69‑1 (MQ=255) gTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt < 1:1891519/68‑1 (MQ=255) tCGTAATCCATTTGGCGCATTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:3110899/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCgataa > 1:639376/1‑56 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:805312/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:1007219/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:806312/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:2858902/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:2977784/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:2102391/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGc > 1:693202/1‑68 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1661884/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1217487/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:2962849/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:2765993/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:2638214/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:2448284/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1053433/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:3344275/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:220349/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:3386742/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:3430917/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1963835/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1470773/1‑69 (MQ=255) tCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCGTCTTCATCGATAATGCTCATGGCa > 1:1852859/1‑69 (MQ=255) ccATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc < 1:666472/40‑1 (MQ=255) ccATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc < 1:3245242/40‑1 (MQ=255) ccATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc < 1:886074/40‑1 (MQ=255) tGGCGCAGTGCGTCTAATGTGGCTTTTGCTTCATCTTCGTCGATGATGCt < 1:1392458/50‑1 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc > 1:3083184/1‑69 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:980342/69‑1 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:1329656/69‑1 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2338961/69‑1 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2318728/69‑1 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc > 1:1868867/1‑69 (MQ=255) gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2284250/69‑1 (MQ=255) cGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:648577/1‑69 (MQ=255) cGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2230974/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt > 1:2199543/1‑36 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACt > 1:1692064/1‑68 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2837271/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:1549436/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:666968/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:1423202/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:462654/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3253688/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3266060/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2338806/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3274768/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:80651/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2564716/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:830087/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2337516/1‑69 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCGTGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:2922619/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:3308715/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:166315/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:714501/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1787740/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:95305/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:124352/1‑68 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:1663803/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3017610/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:218259/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2301115/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:693106/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2852910/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2908981/69‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:717200/68‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:1855410/68‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3145728/68‑1 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1330846/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1408852/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:613511/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:3206768/1‑63 (MQ=255) tggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3230100/67‑1 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGAtg > 1:929938/1‑68 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:994772/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:3321076/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:3316086/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:2999074/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:2458368/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:1816961/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:1010172/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCAGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:68171/1‑69 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:361274/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:2731376/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:2323532/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:1767798/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1451476/63‑1 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1408976/63‑1 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:1227390/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTAATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1781644/63‑1 (MQ=255) | GGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATC > minE/2002485‑2002598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |