| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,002,578 | C→T | 24.2% | V49V (GTG→GTA) | hybG ← | hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,002,578 | 0 | C | T | 24.2% | 105.6 / 26.0 | 82 | V49V (GTG→GTA) | hybG | hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base C (44/18); new base T (9/11); total (53/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAT > minE/2002513‑2002646 | gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc > 1:1868867/1‑69 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2338961/69‑1 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2318728/69‑1 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:1329656/69‑1 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc > 1:3083184/1‑69 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:2284250/69‑1 (MQ=255)gCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAcc < 1:980342/69‑1 (MQ=255) cGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2230974/1‑69 (MQ=255) cGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:648577/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACt > 1:1692064/1‑68 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2337516/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3253688/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3266060/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:3274768/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2338806/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2564716/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:2837271/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:830087/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:666968/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:1423202/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:80651/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:1549436/1‑69 (MQ=255) tAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTg > 1:462654/1‑69 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCGTGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:2922619/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:166315/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:714501/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:124352/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:95305/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:3308715/1‑68 (MQ=255) tggctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1787740/1‑68 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:218259/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2852910/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2908981/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:2301115/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3017610/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:693106/69‑1 (MQ=255) tttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:1663803/69‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:1855410/68‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3145728/68‑1 (MQ=255) ttggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:717200/68‑1 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:3206768/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:613511/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1408852/1‑63 (MQ=255) ttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc > 1:1330846/1‑63 (MQ=255) tggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCcagcag < 1:3230100/67‑1 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGAtg > 1:929938/1‑68 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:1010172/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:3316086/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:3321076/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:994772/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:2458368/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:2999074/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:1816961/1‑69 (MQ=255) ggcttCGTCTTCATCGATAATGCTCATGGCAAATCAGACGTGTACCAGTACCCACTGGCCCAGCAGATc > 1:68171/1‑69 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:2731376/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1408976/63‑1 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:2323532/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:1227390/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1451476/63‑1 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:361274/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg > 1:1767798/1‑63 (MQ=255) ttCATCTTCGTCGATGATGCTAATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:1781644/63‑1 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2141779/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:16005/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2184311/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:361782/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2206178/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:518190/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:588465/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:2338113/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:1375576/1‑44 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCAccc > 1:3010583/1‑44 (MQ=255) cgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:723996/52‑1 (MQ=255) cATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCa < 1:812984/68‑1 (MQ=255) cATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCa < 1:3135624/68‑1 (MQ=255) cATGGCAAATACGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCa < 1:2287120/68‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:998917/69‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:1030560/69‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:3374041/69‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:2985598/69‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:1835443/69‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:107764/69‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:340269/69‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:2733101/69‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:1406846/69‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCAAAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:1365199/69‑1 (MQ=255) | GCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAT > minE/2002513‑2002646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |