| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,002,764 | C→T | 27.0% | L105L (CTG→CTA) | hybF ← | protein involved with the maturation of hydrogenases 1 and 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,002,764 | 0 | C | T | 27.0% | 119.1 / 40.2 | 103 | L105L (CTG→CTA) | hybF | protein involved with the maturation of hydrogenases 1 and 2 |
| Reads supporting (aligned to +/- strand): ref base C (47/28); new base T (15/13); total (62/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
ACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTGCGCA > minE/2002711‑2002821 | aCGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTa > 1:913565/1‑69 (MQ=255)aCGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTa > 1:631180/1‑69 (MQ=255) cGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGt < 1:2823506/67‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:152550/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:925139/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:155327/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:159514/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:79707/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:1722441/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:1928253/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg < 1:2234414/49‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:2526491/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:2738869/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:3001179/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:303044/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:3181246/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg < 1:3235483/49‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg < 1:558287/49‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg < 1:554380/49‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:1332596/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:1384605/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:1154913/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCg > 1:848901/1‑49 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACg > 1:3369920/1‑68 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:3253638/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:3287564/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2535056/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2569595/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2502626/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2582977/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:868584/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:1102398/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2707871/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:1217114/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:3348788/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:1310029/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:2773340/69‑1 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:1897620/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:66705/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:1114050/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:2927644/1‑69 (MQ=255) cacaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc > 1:1564286/1‑69 (MQ=255) acaTTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:1719602/68‑1 (MQ=255) attattCACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:2892/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCTGGTATc > 1:3075269/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:2921727/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:713346/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:22028/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:1446533/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:1373094/1‑59 (MQ=255) attattAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATc > 1:3430585/1‑59 (MQ=255) attaACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGc > 1:1091945/1‑69 (MQ=255) attaACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGc > 1:833382/1‑69 (MQ=255) aaCTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGa > 1:2307184/1‑41 (MQ=255) aaCTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGa > 1:100101/1‑41 (MQ=255) aaCTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGa > 1:2776605/1‑41 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:2059084/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:2886337/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:112002/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:1607653/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:470714/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:750745/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:2608113/69‑1 (MQ=255) ccGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGt < 1:2572441/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:366711/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:1084478/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:3287607/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:592671/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2676509/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2563282/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2539790/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2530891/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:1248302/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:796098/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2173091/69‑1 (MQ=255) tATTCAACTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2135837/69‑1 (MQ=255) aaCTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGc < 1:2773342/46‑1 (MQ=255) cTTCAATACTTTTGACGATTAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCATGACag < 1:2869642/62‑1 (MQ=255) cTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGac < 1:2882332/69‑1 (MQ=255) cTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGac < 1:1679598/69‑1 (MQ=255) cTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGac < 1:1687886/69‑1 (MQ=255) ttCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGaca < 1:1350485/69‑1 (MQ=255) ttCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGaca < 1:1150909/69‑1 (MQ=255) ttCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGaca < 1:164303/69‑1 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:2583795/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:3407278/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:3403083/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:3126471/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:2840839/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:2791629/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:2185277/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:1682600/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:1612051/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:1482262/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:12744/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGacac > 1:1061326/1‑39 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCCGGTATCGaca > 1:3411605/1‑38 (MQ=255) cAATACTTTTGACGATTAGCGAATCGCAGGTATCGacac > 1:2891127/1‑39 (MQ=38) cTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTGCGCa > 1:2724612/1‑69 (MQ=255) cTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTGCGCa > 1:1132576/1‑69 (MQ=255) | ACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTGCGCA > minE/2002711‑2002821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |