| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 161,135 | C→T | 21.1% | intergenic (+2/‑153) | degP → / → cdaR | serine endoprotease (protease Do), membrane‑associated/DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 161,135 | 0 | C | T | 21.1% | 200.3 / 37.9 | 136 | intergenic (+2/‑153) | degP/cdaR | serine endoprotease (protease Do), membrane‑associated/DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (76/31); new base T (6/23); total (82/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
GCAAACCGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCA > minE/161066‑161203 | gCAAACCGTCTGT‑CTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAAtc < 1:1194525/69‑1 (MQ=255) cAAACCGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAAtc < 1:2574641/69‑1 (MQ=255) cAAACCGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAAtc < 1:1514779/69‑1 (MQ=255) cGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTccc > 1:2587637/1‑68 (MQ=255) cGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTccc > 1:1332033/1‑68 (MQ=255) cACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTcc > 1:1051376/1‑68 (MQ=255) cACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTcc > 1:1122356/1‑68 (MQ=255) cACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTcc > 1:124439/1‑68 (MQ=255) cACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTcc > 1:892915/1‑68 (MQ=255) cACTCAACATTCAGCGCGGCGACAGCACCATATACCTGTTAATGCAGTAATCTCCATCAACCCCTTcc > 1:2264615/1‑68 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTc > 1:1931656/1‑54 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTc > 1:1695419/1‑54 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTc > 1:1532941/1‑54 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTc > 1:786462/1‑54 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTc > 1:2848433/1‑54 (MQ=255) cAACATTCAGCGCGGCGACAGCACCATCTACCTGATAATGCAGTAATCTCCCTc > 1:1028147/1‑54 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:932696/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:220364/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:1593242/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:3208150/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:20345/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:1761728/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:3419909/69‑1 (MQ=255) ttCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg < 1:3368176/69‑1 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAAc > 1:2375318/1‑64 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:2387405/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:2887928/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:3269256/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:559469/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:71805/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:136360/1‑67 (MQ=255) cAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:1253156/1‑67 (MQ=255) cAGCGCGGCGAAAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACggg > 1:1402505/1‑67 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:3273795/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:3407605/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:375460/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:993631/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:56385/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:2636547/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:829481/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:944893/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:980965/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:2468646/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:1282747/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:1290547/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:1845454/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:1203353/68‑1 (MQ=255) gcgcGGCGACAGCACCATCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:189843/68‑1 (MQ=255) cgcgGCGACAGCACCATCTTCCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAg < 1:2875712/67‑1 (MQ=255) aCCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTAc > 1:2362864/1‑68 (MQ=255) aCCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTAc > 1:1129993/1‑68 (MQ=255) aCCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACa > 1:2539572/1‑69 (MQ=255) aCCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACa > 1:3120628/1‑69 (MQ=255) aCCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACa > 1:1721905/1‑69 (MQ=255) aTCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATc < 1:240230/69‑1 (MQ=255) aTCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATc < 1:1879875/69‑1 (MQ=255) aTCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATc < 1:911731/69‑1 (MQ=255) aTCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATc < 1:3233612/69‑1 (MQ=255) tCTACCTGTTAATGCAGTAATTTCCGTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAAt > 1:1590198/1‑67 (MQ=255) tCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCt > 1:2472658/1‑69 (MQ=255) tCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCt > 1:2923317/1‑69 (MQ=255) tCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCt > 1:2215290/1‑69 (MQ=255) tCTACCTGTTAATGCAGTAATTTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCt > 1:2784830/1‑69 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGt > 1:2528645/1‑53 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:2089241/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:1877341/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:3031763/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:3052227/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:231445/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:664328/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:3161257/1‑55 (MQ=255) tACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTtc > 1:1537727/1‑55 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgtg > 1:550318/1‑66 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgtg > 1:3200839/1‑66 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgtg > 1:310471/1‑66 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgtg > 1:2946964/1‑66 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgtg > 1:2761753/1‑66 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCtgt > 1:3422028/1‑65 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGa < 1:1470108/67‑1 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGa < 1:829207/67‑1 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGa < 1:2758163/67‑1 (MQ=255) tGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGa < 1:1633189/67‑1 (MQ=255) cAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTcaccac < 1:3097566/69‑1 (MQ=255) cAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTcaccac < 1:1306583/69‑1 (MQ=255) cAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACATcaccac < 1:2768675/69‑1 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:107433/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:515253/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:3430700/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:1994568/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:2974341/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:2920013/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:282444/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:263783/1‑69 (MQ=255) aTCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGGTCTCCTTACAATCTGTGAACTTCACCACAACTc > 1:2474168/1‑69 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATc < 1:2467849/47‑1 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACTACTCCa < 1:437123/69‑1 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCa < 1:2082406/69‑1 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCa < 1:3182586/69‑1 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCa < 1:3174978/69‑1 (MQ=255) ctcCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCa < 1:26727/69‑1 (MQ=255) | GCAAACCGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAATCTCCCTCAACCCCTTCCTGAAAACGGGAAGGGGTTCTCCTTACAATCTGTGAACTTCACCACAACTCCA > minE/161066‑161203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |