| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,004,772 | G→T | 36.4% | G326G (GGC→GGA) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,004,772 | 0 | G | T | 36.4% | 88.0 / 69.4 | 96 | G326G (GGC→GGA) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/28); new base T (4/31); total (37/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01 | |||||||||||
GTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCATT > minE/2004704‑2004834 | gtggtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATg > 1:3244030/1‑69 (MQ=255)gtggtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATg > 1:2925050/1‑69 (MQ=255)gtggtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATg > 1:2880988/1‑69 (MQ=255) ccTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg < 1:2902299/69‑1 (MQ=255) tGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTc > 1:3000248/1‑69 (MQ=255) gCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa < 1:1048737/69‑1 (MQ=255) gCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa < 1:2794460/69‑1 (MQ=255) gCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa < 1:2342933/69‑1 (MQ=255) gCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa > 1:223352/1‑69 (MQ=255) gCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa < 1:2202425/69‑1 (MQ=255) gcggcgCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCgg < 1:3417239/69‑1 (MQ=255) gcggcgCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCgg < 1:882088/69‑1 (MQ=255) gcggcgCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCgg < 1:1720432/69‑1 (MQ=255) gcgcTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGa < 1:3279744/67‑1 (MQ=255) gcgcTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGa < 1:2257632/67‑1 (MQ=255) gcgcTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGa < 1:801478/67‑1 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:2351130/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:3358068/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:3045030/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:2543018/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATgaga > 1:3029794/1‑68 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:1023177/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:2258050/1‑69 (MQ=255) tCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGaa > 1:1558994/1‑69 (MQ=255) cGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCa < 1:846055/56‑1 (MQ=255) cGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCgg < 1:2349832/60‑1 (MQ=255) cTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTg < 1:1487692/68‑1 (MQ=255) tttATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGtt < 1:187034/67‑2 (MQ=255) tataCCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCg > 1:2532770/1‑69 (MQ=255) tataCCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCg > 1:86041/1‑69 (MQ=255) tataCCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCg > 1:833279/1‑69 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2652404/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2747587/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2527284/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2410598/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:1528982/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:1084793/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2282996/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:819812/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:1081557/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:2089178/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:498446/1‑68 (MQ=255) gTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACg > 1:64544/1‑68 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:2586371/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:300676/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:710422/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:1144213/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:2870242/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:653507/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:456992/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:2101247/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:21957/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:3100258/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAg > 1:472566/1‑69 (MQ=255) tGCTTCGCGCTTTCCTGAATGCCATTGATCAGGTATTCATCgg > 1:1089587/1‑43 (MQ=255) tCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGtt < 1:183067/50‑2 (MQ=255) tttCCTGAATTCCTTTGATCATGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2552685/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:3364075/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:989716/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:896180/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:3434336/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:385665/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:465961/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:540274/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:560855/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:641423/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:694102/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:709661/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:3234267/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1280553/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1307439/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1405017/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1438349/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1566097/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1635602/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1666154/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2211518/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2273894/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2344673/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:1117142/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:326932/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2751489/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2901259/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:3108792/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:3202973/60‑1 (MQ=255) tttCCTGAATTCCTTTGATCAGGCATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:856310/60‑1 (MQ=255) tttCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCg < 1:2341721/69‑1 (MQ=255) tttCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCg < 1:527095/69‑1 (MQ=255) ttCCTGAATTCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAgg < 1:2338377/59‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:1141613/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:117100/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:853561/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:109694/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:149255/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:709918/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:2028491/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:2377985/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:2660959/68‑1 (MQ=255) tGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCAtt < 1:456933/68‑1 (MQ=255) | GTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGATCGGACGATACGAGGACAGATCCGCATT > minE/2004704‑2004834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |