| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,004,952 | G→A | 26.3% | A266A (GCC→GCT) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,004,952 | 0 | G | A | 26.3% | 77.2 / 24.4 | 68 | A266A (GCC→GCT) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/36); new base A (6/12); total (20/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
TTCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGTTTGTCGATGAAAGA > minE/2004889‑2005017 | ttCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAAt < 1:681748/69‑1 (MQ=255)ttCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAAt < 1:2713024/69‑1 (MQ=255)ttCCGGCACGCTCAGGCAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAAt < 1:2954267/69‑1 (MQ=255) tCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATa < 1:2136456/69‑1 (MQ=255) tCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATa < 1:3144859/69‑1 (MQ=255) ccGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATa < 1:2699665/68‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1128247/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1099036/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1824446/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1809579/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1716324/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:1620580/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:14598/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:576341/69‑1 (MQ=255) gTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCAGGGTAGAACGCGGCAATAACTGCGGTATCAAc < 1:2995742/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2981779/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2845334/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2805585/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:3297834/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:3348679/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:402036/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2493082/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2647577/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:2013427/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:1711127/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:1769668/69‑1 (MQ=255) aGTTCACCGCACCTTTACCGCGCGTCAGCCATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCt < 1:1446477/69‑1 (MQ=255) gTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCt > 1:1642217/1‑68 (MQ=255) gTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCt > 1:18617/1‑68 (MQ=255) tttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:3134356/1‑69 (MQ=255) tttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:522012/1‑69 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTca < 1:1943969/69‑1 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTca < 1:2082039/69‑1 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:3082179/1‑68 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:3040105/1‑68 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:1537764/1‑68 (MQ=255) ttACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTc > 1:2923365/1‑68 (MQ=255) gcgTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTAAACaaa < 1:4214/69‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:1196647/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:606306/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:619481/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:1465276/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa > 1:1144006/1‑42 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:684557/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:1940135/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:2875406/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:2092575/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa > 1:1784392/1‑42 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa > 1:2099435/1‑42 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:2364995/42‑1 (MQ=255) tCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCaa < 1:2904076/42‑1 (MQ=255) aGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTc > 1:1505399/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTCAg > 1:939371/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTCAg > 1:2857288/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTCAg > 1:279401/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTCAg > 1:412944/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTCAg > 1:558746/1‑69 (MQ=255) ccATTCCGGGTAGAACGCAGCGATAACTGCAGTATCAACCTTGTAAACCTGCTCAACAAAGTCGCTAAg > 1:660698/1‑69 (MQ=255) aTTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt > 1:2629886/1‑69 (MQ=255) aTTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt > 1:1531582/1‑69 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTTCTCAACAAAGTCGCTCAGtt < 1:967186/61‑1 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:1868856/61‑1 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:564773/61‑1 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:1898696/61‑1 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:1449322/61‑1 (MQ=255) tAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:2730785/61‑1 (MQ=255) aGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:3397203/60‑1 (MQ=255) aGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGtt < 1:2060273/60‑1 (MQ=255) cgcgGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGTTTGTCGATGAAAGa < 1:183095/69‑1 (MQ=255) | TTCCGGCACGCTCAGGTAGTTCACCGCACCTTTACCGCGTGTCAGCCATTCCGGGTAGAACGCGGCAATAACTGCGGTATCAACCTTATAAACCTGCTCAACAAAGTCGCTCAGTTTGTCGATGAAAGA > minE/2004889‑2005017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |