| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,006,358 | C→T | 57.7% | G189G (GGG→GGA) | hybB ← | predicted hydrogenase 2 cytochrome b type component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,006,358 | 0 | C | T | 57.7% | 24.9 / 63.7 | 85 | G189G (GGG→GGA) | hybB | predicted hydrogenase 2 cytochrome b type component |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base T (35/14); total (51/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
CAGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACC > minE/2006292‑2006421 | cAGCGGCAACGTTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1931734/69‑1 (MQ=255)cAGCGGCAACATTTCATAGCTCTGTCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1664553/69‑1 (MQ=255) aGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:1130094/68‑1 (MQ=255) aGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGAccc < 1:3010105/68‑1 (MQ=255) aaCATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGaa < 1:431581/69‑1 (MQ=255) aaCATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGaa < 1:1358944/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGTTCATTAGCGACCCCATTGAAGACTgg < 1:942344/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2921011/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:229705/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2833842/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:1251173/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:941404/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:982879/69‑1 (MQ=255) tCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:2571759/69‑1 (MQ=255) cATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTgg < 1:1427328/68‑1 (MQ=255) aTGCACCTTGTAGCCCGCCTAGATCATCAGCGATCCCa > 1:2794896/1‑38 (MQ=38) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGAtccc > 1:769297/1‑37 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2185736/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1787661/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1835167/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:416548/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:404664/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:401917/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:3220663/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2722577/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:2585026/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1109485/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:679001/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1487700/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:689878/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1288531/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1294363/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGATCCCa > 1:1393433/1‑38 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:1707761/69‑1 (MQ=255) aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:2753730/69‑1 (MQ=255) gCCCGCCTAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCcat > 1:1863398/1‑69 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:2961699/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1045145/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1345899/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1616519/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1644915/69‑1 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGgcag > 1:1777331/1‑41 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2298012/1‑68 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2952348/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:899179/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2911097/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2966846/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:80504/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:584893/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1095666/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1138461/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1734151/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1445248/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1441565/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2267352/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2208772/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1360952/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1985450/1‑69 (MQ=255) ataatcaGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2473503/4‑68 (MQ=255) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3192870/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:157013/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:477388/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:790864/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:795274/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3349989/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:80399/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:8186/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3215600/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3074979/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2018400/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2300039/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2901254/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:287622/37‑1 (MQ=37) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc < 1:402178/69‑1 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1226388/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1193713/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1510496/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:596133/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:591350/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1836348/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1918847/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2595053/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:197059/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:328097/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2160748/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2323968/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2506644/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:258815/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAc > 1:3317296/1‑68 (MQ=255) gCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATtaagaa < 1:1412031/61‑1 (MQ=255) | CAGCGGCAACATTTCATAGCTCTGCCACAACGGATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACC > minE/2006292‑2006421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |