| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 161,956 | T→C | 46.7% | D223D (GAT→GAC) | cdaR → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 161,956 | 0 | T | C | 46.7% | 4.0 / 63.7 | 75 | D223D (GAT→GAC) | cdaR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (24/16); new base C (17/18); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTCGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGC > minE/161899‑162020 | ctcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCagaag > 1:1016952/1‑65 (MQ=255)ctcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCagaag > 1:3144395/1‑65 (MQ=255)ctcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCagaag > 1:2137870/1‑65 (MQ=255)ctcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCagaag > 1:817931/1‑65 (MQ=255)ctcCCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCagaag > 1:2550868/1‑65 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:3208446/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:3399258/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:1857336/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:787374/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:3104148/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:3100105/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:2408084/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:3399572/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:1387265/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:609861/1‑69 (MQ=255) tcGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGatcat > 1:2456012/1‑69 (MQ=255) cGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCg < 1:1307921/69‑1 (MQ=255) tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:2901445/69‑1 (MQ=255) tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:1567778/69‑1 (MQ=255) tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:1563641/69‑1 (MQ=255) tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:470370/69‑1 (MQ=255) ggtggtGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:1239581/68‑1 (MQ=255) ggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaac < 1:1784634/68‑1 (MQ=255) ggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaac < 1:2290957/68‑1 (MQ=255) gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTTGGATGCAGAAGATCATCGTAAGCGAGTTGaacaa < 1:1341022/69‑1 (MQ=255) gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaacaa < 1:3381441/69‑1 (MQ=255) gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaacaa < 1:3113960/69‑1 (MQ=255) gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaacaa < 1:1680750/69‑1 (MQ=255) tgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGaacaa < 1:1491589/68‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:34036/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1182458/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1189785/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1222957/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:875442/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1337875/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:2871501/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1997506/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:395956/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:469266/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:2469862/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:204532/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:2335527/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:2377756/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:639488/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:817154/66‑1 (MQ=255) gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:1149298/66‑1 (MQ=255) ttGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGaaca < 1:3274851/65‑1 (MQ=255) gAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:2916485/1‑69 (MQ=255) gAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:1356623/1‑69 (MQ=255) gAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:699764/1‑69 (MQ=255) gAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:1683283/1‑57 (MQ=255) gAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:1491112/1‑57 (MQ=255) gAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:3351232/1‑57 (MQ=255) gAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGAt > 1:3355222/1‑57 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTGCCCGCa > 1:3107527/1‑65 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCa > 1:1334856/1‑65 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCa > 1:714168/1‑65 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:918250/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:897752/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:3005618/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2754532/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2699703/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2684178/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2498174/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2452618/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2435753/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:2285833/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:1977264/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:1549731/1‑66 (MQ=255) gAACTCTTTTGGGCGCTGGGACGCAGAAGATAATCGTAAGCGAGTTGAACAACTGATTACCCGCAt > 1:3131685/1‑66 (MQ=255) gAACTCTTTTGGGCGCT‑GGACGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGa > 1:1265994/1‑67 (MQ=255) tctTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAagag < 1:1056495/69‑1 (MQ=255) tttGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAagag < 1:1607882/66‑1 (MQ=255) tGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTg < 1:1739761/38‑1 (MQ=255) gggATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGc > 1:1128168/1‑69 (MQ=255) | CTCGCTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGC > minE/161899‑162020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |