| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,006,382 | C→T | 35.5% | P181P (CCG→CCA) | hybB ← | predicted hydrogenase 2 cytochrome b type component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,006,382 | 0 | C | T | 35.5% | 64.0 / 51.1 | 90 | P181P (CCG→CCA) | hybB | predicted hydrogenase 2 cytochrome b type component |
| Reads supporting (aligned to +/- strand): ref base C (29/29); new base T (18/14); total (47/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
ATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTC > minE/2006325‑2006448 | aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:1707761/69‑1 (MQ=255)aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:2753730/69‑1 (MQ=255) gCCCGCCTAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCcat > 1:1863398/1‑69 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1345899/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1616519/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1644915/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1045145/69‑1 (MQ=255) gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:2961699/69‑1 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGgcag > 1:1777331/1‑41 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2298012/1‑68 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2267352/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1095666/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2208772/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:584893/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1985450/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2911097/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1734151/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2952348/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2966846/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1360952/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1138461/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:899179/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:80504/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1445248/1‑69 (MQ=255) atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1441565/1‑69 (MQ=255) ataatcaGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2473503/4‑68 (MQ=255) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3215600/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3349989/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:80399/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2300039/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:8186/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3074979/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3192870/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:287622/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2901254/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:157013/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:477388/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:790864/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2018400/37‑1 (MQ=37) catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:795274/37‑1 (MQ=37) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2595053/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1510496/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1193713/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1836348/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:596133/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1918847/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1226388/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:328097/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc < 1:402178/69‑1 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:258815/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:197059/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2506644/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:591350/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2323968/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2160748/1‑69 (MQ=255) aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAc > 1:3317296/1‑68 (MQ=255) gCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATtaagaa < 1:1412031/61‑1 (MQ=255) gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:656510/1‑69 (MQ=255) gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:25827/1‑69 (MQ=255) gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:1155155/1‑69 (MQ=255) gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:3046225/1‑69 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:301749/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:635449/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2069024/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2655817/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:3314243/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:3353415/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2179907/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:405202/67‑1 (MQ=255) gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:474401/67‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:2140330/69‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:1988584/69‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:563337/69‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:2420345/69‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:3263811/69‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:2255329/68‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:986471/68‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:1314024/68‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:3196616/68‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:1857192/68‑1 (MQ=255) gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:2806253/68‑1 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:1837560/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:1459064/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:1457206/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:523858/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:123812/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:1234585/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:2824776/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:952732/1‑69 (MQ=255) gTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTc > 1:453550/1‑69 (MQ=255) | ATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACACCTTC > minE/2006325‑2006448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |