| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,007,036 | T→C | 31.5% | I289V (ATT→GTT) ‡ | hybA ← | hydrogenase 2 4Fe‑4S ferredoxin‑type component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,007,036 | 0 | T | C | 31.5% | 80.7 / 47.8 | 95 | I289V (ATT→GTT) ‡ | hybA | hydrogenase 2 4Fe‑4S ferredoxin‑type component |
| Reads supporting (aligned to +/- strand): ref base T (44/21); new base C (19/11); total (63/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACAC > minE/2006976‑2007104 | cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTctg < 1:910981/69‑3 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTc > 1:3384911/1‑67 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTc > 1:1772431/1‑67 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1348982/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3315969/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3320038/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1645665/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:155206/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:427512/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:597354/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:784762/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1982066/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:1408643/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:826113/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2069923/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:865440/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2174470/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2994149/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:105606/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:235211/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2421767/69‑1 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2709212/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2614432/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2600987/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTAAACAGGGTGTGTTGGACATGTTCgg > 1:109026/1‑69 (MQ=255)cGGTTAAGCCCGCCAGCACAGCCAGT‑GTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCGGa > 1:1966919/1‑69 (MQ=255) ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:324552/68‑1 (MQ=255) ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3042716/68‑1 (MQ=255) ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2365528/68‑1 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1124058/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:3175634/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1890557/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1892838/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:889957/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:424940/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1391043/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1294219/1‑69 (MQ=255) tAAGCCCGCCAGCACAGCCAGTGGAAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:2214784/1‑69 (MQ=255) cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTc > 1:404893/1‑51 (MQ=255) cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:1856500/1‑67 (MQ=255) cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:2180853/1‑67 (MQ=255) aGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAg < 1:1217913/69‑1 (MQ=255) gTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAg < 1:1953281/64‑1 (MQ=255) gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:955446/1‑69 (MQ=255) gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:2331771/1‑69 (MQ=255) gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:79026/1‑69 (MQ=255) atGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:1150134/1‑48 (MQ=255) atGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGc > 1:286309/1‑69 (MQ=255) gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:299472/69‑1 (MQ=255) gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:122617/69‑1 (MQ=255) gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:1090773/69‑1 (MQ=255) gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:2609215/69‑1 (MQ=255) tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:884816/1‑69 (MQ=255) tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:2984889/1‑69 (MQ=255) tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:2420142/1‑69 (MQ=255) tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:1735054/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:302880/1‑43 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:3062999/1‑43 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:3127181/1‑43 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:925769/1‑43 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAt > 1:2240079/1‑59 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaaag < 1:2799241/69‑3 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1419154/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1764248/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2533203/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3323726/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:163398/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1540040/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:153832/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:644939/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1475527/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1718824/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1354870/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1285003/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1272321/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1204832/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1113145/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1089899/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:2484651/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3131671/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2440496/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:2949304/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2202648/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1780045/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:198313/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCAAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3124878/1‑69 (MQ=255) ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGACCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:640484/69‑1 (MQ=255) ttGAATATTTTCGGAACGCGCACCAGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2221693/1‑69 (MQ=255) ttGAATATTTTCCGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGTCAGGTCGAGATTTTCATAAgg > 1:2256112/1‑69 (MQ=255) gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGTca < 1:2053138/69‑1 (MQ=255) gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1569817/69‑1 (MQ=255) gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:399343/69‑1 (MQ=255) gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1680641/69‑1 (MQ=255) gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1128442/69‑1 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGcaca < 1:609750/68‑1 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGcaca < 1:2094164/68‑1 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGcaca > 1:2215148/1‑68 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGcaca < 1:1939125/68‑1 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGcaca < 1:1730204/68‑1 (MQ=255) tatTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCacac > 1:2676961/1‑69 (MQ=255) | CGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACAC > minE/2006976‑2007104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |