| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,008,176 | A→G | 46.8% | G282G (GGT→GGC) | hybO ← | hydrogenase 2, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,008,176 | 0 | A | G | 46.8% | 3.7 / 69.1 | 79 | G282G (GGT→GGC) | hybO | hydrogenase 2, small subunit |
| Reads supporting (aligned to +/- strand): ref base A (28/14); new base G (16/21); total (44/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
ATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCC > minE/2008123‑2008238 | aTACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg > 1:829701/1‑69 (MQ=255)aTACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg > 1:2654566/1‑69 (MQ=255)aTACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg > 1:2728930/1‑69 (MQ=255)aTACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg > 1:178155/1‑69 (MQ=255)aTACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg > 1:3036704/1‑69 (MQ=255) aCCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGaa > 1:2235682/1‑69 (MQ=255) aCCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGaa > 1:1312827/1‑69 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACGGCCAACATCGCAGAATTGCAGCGTTGAGCAGt > 1:1010087/1‑69 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAg > 1:695100/1‑68 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGt > 1:1187183/1‑69 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGt > 1:1202002/1‑69 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGt > 1:2315981/1‑69 (MQ=255) cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGt > 1:501106/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCa > 1:1526307/1‑39 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAAc > 1:2303704/1‑41 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAg > 1:1008856/1‑63 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTg > 1:1616056/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTg > 1:510252/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTg > 1:2912498/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTg > 1:2815480/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTg > 1:2333969/1‑69 (MQ=255) tAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGAAGCGTTGAGCAGTTg > 1:3374013/1‑69 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:936828/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:417362/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:750589/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:2321604/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:245906/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:71245/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:564284/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:553167/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:300390/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:3035658/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:3076335/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:2195631/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:1494723/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:1550249/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGc > 1:168711/1‑68 (MQ=255) gCACGGGTGACCAATCGCTACCGGCCACACGCCGCCAAAATCGCAGAATTGCAg > 1:1489042/1‑54 (MQ=37) gTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGt > 1:2031978/1‑69 (MQ=255) gTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGt > 1:1190554/1‑69 (MQ=255) tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGtt < 1:3257520/69‑1 (MQ=255) tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGtt > 1:523274/1‑69 (MQ=255) tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGtt > 1:2203855/1‑69 (MQ=255) tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGtt < 1:1709206/69‑1 (MQ=255) tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGtt > 1:874605/1‑69 (MQ=255) cAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTg > 1:299444/1‑69 (MQ=255) tCGCCACCGTCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:299828/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTTAGCAGTTGCCGTAAGTTTCTGGcc < 1:391241/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:1827134/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:211452/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:506863/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:1653375/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:1557087/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:3065798/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:1290936/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:2551444/69‑1 (MQ=255) tCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGCTTCTGGcc < 1:2783121/69‑1 (MQ=255) gCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGcc < 1:44915/67‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTTCCGTAAGTTTCTGGCCCTTTACAGcc < 1:755111/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1320940/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1303024/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1267886/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:878809/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:761152/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:772213/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1134344/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1560257/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:3208749/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:3123875/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:3108044/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1576528/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1641657/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1644430/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:2849950/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:1835991/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:2513632/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:2503476/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:2371110/69‑1 (MQ=255) ccACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGcc < 1:2340056/69‑1 (MQ=255) | ATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCC > minE/2008123‑2008238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |