| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,008,326 | G→A | 100% | C232C (TGC→TGT) | hybO ← | hydrogenase 2, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,008,326 | 0 | G | A | 81.7% | 54.8 / 5.4 | 38 | C232C (TGC→TGT) | hybO | hydrogenase 2, small subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/1); new base A (15/16); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGACGGCCATAGGCGAAGGTCGGACGGTTTTTGTC > minE/2008273‑2008376 | tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2200853/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:3418798/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:356216/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:447611/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2955458/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2618627/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2584995/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2287363/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2057804/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2053902/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:601788/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:2005836/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:1783154/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:669552/69‑1 (MQ=255)tcatcaCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:1779154/69‑1 (MQ=255) caCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGAc > 1:214639/1‑69 (MQ=255) caCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGAc > 1:2220673/1‑69 (MQ=255) caCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGAc > 1:611397/1‑69 (MQ=255) caCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGAc > 1:1635985/1‑69 (MQ=255) aCCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGACg > 1:2707066/1‑69 (MQ=255) aaCTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGACGGCCa < 1:454048/69‑1 (MQ=255) ctctTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGACGGCCAt > 1:530328/1‑68 (MQ=255) aaaCGACCAGCATCGAAGTGCGGGCGACGTTCACAGTGTTCGTGAATc < 1:1170212/48‑1 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGt > 1:2509324/1‑68 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:474994/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:514692/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:1022966/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:844139/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:3248583/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:3182209/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:2956789/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:2525967/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:249643/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:2113292/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:1918635/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTCGGACGGTTTTTGTc > 1:1262780/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGTGTTCGTGAATCAGACGACCATAGGCGAAAGTAGGACGGTTTTTGTc > 1:14325/1‑69 (MQ=255) gAAGTGCGGGCGACGTTCACAGGGTTCGTGAATCAGACGACCATAGGCGAAAGTCgg > 1:1378699/1‑57 (MQ=255) | TCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGTTCGCAGTGTTCGTGAATCAGACGGCCATAGGCGAAGGTCGGACGGTTTTTGTC > minE/2008273‑2008376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |