| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,009,730 | G→T | 61.2% | pseudogene (297/411 nt) | yghX ← | ECK2993:JW5926:b2999; predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,009,730 | 0 | G | T | 61.2% | 9.5 / 26.1 | 31 | pseudogene (297/411 nt) | yghX | ECK2993:JW5926:b2999; predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base T (13/6); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
GATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTA > minE/2009663‑2009777 | gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:1004800/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:805544/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:1057032/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:2112248/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:3127522/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:310641/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:3038228/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:2804455/69‑1 (MQ=255)gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:2773690/69‑1 (MQ=255)gATCGGCGGCAGAATTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga < 1:2617725/69‑1 (MQ=255) aTCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga > 1:3249171/1‑68 (MQ=255) aTCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga > 1:2255096/1‑68 (MQ=255) aTCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgataga > 1:1964586/1‑68 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:2223638/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:1033079/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:2380196/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:2586206/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:2946119/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:3426786/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:355892/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:1075069/69‑1 (MQ=255) tCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGgatagata < 1:1370830/69‑1 (MQ=255) aTTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCAt < 1:1035614/69‑1 (MQ=255) ttGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATag < 1:3159541/69‑2 (MQ=255) ttGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATag < 1:1160618/69‑2 (MQ=255) ttGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATag < 1:1560260/69‑2 (MQ=255) ttGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATag < 1:3084876/69‑2 (MQ=255) tGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATag < 1:2155894/68‑2 (MQ=255) tCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAAc < 1:2088648/68‑1 (MQ=255) tCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAAc < 1:610425/68‑1 (MQ=255) tCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAAc < 1:599990/68‑1 (MQ=255) aTAACGGGGCGTGGAATCATTATGTAATCCGTGATTAACCCCCGgatagata < 1:1152709/52‑1 (MQ=255) aCGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCttatt < 1:516251/69‑1 (MQ=255) aCGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCttat < 1:371647/68‑1 (MQ=255) ggggCGTGGAGTCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:759964/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:1096500/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:2082063/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:795296/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:674882/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:2389990/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:2484694/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:454117/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:322945/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:314336/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:3133064/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:192212/1‑69 (MQ=255) ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATATATATACGCCTCATAGACCTtattat > 1:1544170/1‑69 (MQ=255) gCGTGGAATCATTATGGAATCCGTGATTAACCCCCGTATAGatat > 1:698216/1‑45 (MQ=255) gCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTgc > 1:2046916/1‑68 (MQ=255) gAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTc < 1:2079000/69‑1 (MQ=255) cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTa > 1:297925/1‑67 (MQ=255) cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTa > 1:2709200/1‑67 (MQ=255) cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTa > 1:1388606/1‑67 (MQ=255) cGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTAAACGCCGCCTCGTa > 1:3419452/1‑67 (MQ=255) | GATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTA > minE/2009663‑2009777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |