| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,009,916 | A→T | 43.5% | pseudogene (111/411 nt) | yghX ← | ECK2993:JW5926:b2999; predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,009,916 | 0 | A | T | 43.5% | 21.6 / 64.0 | 62 | pseudogene (111/411 nt) | yghX | ECK2993:JW5926:b2999; predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (16/19); new base T (19/8); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
CCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCG > minE/2009849‑2009983 | ccACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACa > 1:1160555/1‑68 (MQ=255)ccACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAg > 1:1036146/1‑69 (MQ=255)ccACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAg > 1:1574477/1‑69 (MQ=255)ccACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAg > 1:1015727/1‑69 (MQ=255)ccACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAg > 1:3390948/1‑69 (MQ=255) tCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccgcc > 1:1364156/1‑69 (MQ=255) tCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccgcc > 1:2114490/1‑69 (MQ=255) tCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccgcc > 1:378099/1‑69 (MQ=255) tCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAgccgcc > 1:895654/1‑69 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTc < 1:953306/67‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTc < 1:1227533/67‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTc < 1:2702948/67‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTc < 1:476323/67‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:2669978/69‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:3315177/69‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:1497989/69‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:1947348/69‑1 (MQ=255) gggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:2252645/69‑1 (MQ=255) ggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGAt > 1:3380699/1‑69 (MQ=255) ggTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGAt > 1:1585629/1‑69 (MQ=255) acgacCTTAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTc < 1:2340077/58‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:2579099/69‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:2912647/69‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:2949205/69‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:1780203/69‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:1381936/69‑1 (MQ=255) ccATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:401526/69‑1 (MQ=255) cATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:683938/68‑1 (MQ=255) aaaCGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGc > 1:3383086/1‑68 (MQ=255) aaaCGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGc > 1:1023712/1‑68 (MQ=255) aaaCGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGc > 1:942607/1‑68 (MQ=255) aCGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCaa < 1:1437565/68‑1 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGGAAAATcc > 1:2250304/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:3010789/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:405478/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2589361/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:206417/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2156744/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2272008/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:911269/1‑66 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGt < 1:2935195/69‑1 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGt < 1:2097641/69‑1 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGt < 1:3335104/69‑1 (MQ=255) cgcgCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGt < 1:1049247/69‑1 (MQ=255) gcgcAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGt > 1:369885/1‑68 (MQ=255) ggCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGa < 1:485490/36‑1 (MQ=255) ttCCGGATACGCGACTGCTGCCGCGTTCGATACGCCCCCGCCa < 1:451315/43‑1 (MQ=255) ccGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGc > 1:1257310/1‑69 (MQ=255) ccGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGc > 1:1111753/1‑69 (MQ=255) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATATCAAAATcc > 1:1326441/1‑44 (MQ=25) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAAt > 1:9762/1‑42 (MQ=37) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:298104/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:536393/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2839332/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:781017/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2620553/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2386801/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:2257189/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:1166805/1‑44 (MQ=38) gcgACTGCTGCCGCGTTCGATACGCCCCCGCCATAGCAAAATcc > 1:1079669/1‑44 (MQ=38) cAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCg < 1:354797/69‑1 (MQ=255) cAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCg < 1:2878955/69‑1 (MQ=255) cAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCg < 1:653060/69‑1 (MQ=255) | CCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCG > minE/2009849‑2009983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |