| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 162,223 | G→A | 45.2% | A312A (GCG→GCA) | cdaR → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 162,223 | 0 | G | A | 45.2% | 5.4 / 30.5 | 42 | A312A (GCG→GCA) | cdaR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (20/3); new base A (6/13); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACAA > minE/162160‑162288 | aCCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGc > 1:1052497/1‑68 (MQ=255)aCCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGc > 1:1862797/1‑68 (MQ=255)aCCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGc > 1:3381898/1‑68 (MQ=255)aCCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGc > 1:1487679/1‑68 (MQ=255) ttACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACg > 1:482609/1‑69 (MQ=255) ttACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACg > 1:1000042/1‑69 (MQ=255) aCTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGa > 1:291111/1‑68 (MQ=255) aCTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGATGAAAACGa > 1:2063285/1‑68 (MQ=255) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:699573/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:1525687/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:307219/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2138601/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2840280/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2750812/1‑69 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTTAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:704628/67‑1 (MQ=255) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:67224/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:3390959/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2832687/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2960168/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2857349/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2290912/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:197122/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:1908447/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:1289811/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2771442/67‑1 (MQ=39) ggCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2448465/66‑1 (MQ=39) ccAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGcg < 1:234220/61‑1 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTTAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt < 1:923367/69‑1 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt > 1:1976369/1‑69 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt < 1:3055377/69‑1 (MQ=255) aaCGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2880726/62‑1 (MQ=38) gAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTggcg > 1:631234/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGcgacg > 1:1736110/1‑56 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1611935/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:522211/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:605677/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1702766/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1324543/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:3138386/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:780534/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:306036/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:95542/1‑69 (MQ=255) gcgGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACaa > 1:1418348/1‑66 (MQ=255) | ACCTGTGTTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACAA > minE/162160‑162288 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |