| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,010,544 | A→G | 13.6% | intergenic (‑165/‑113) | yghY ← / → yghZ | ECK2994:JW5496:b3000; predicted dienlactone hydrolase/aldo‑keto reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,010,544 | 0 | A | G | 13.6% | 152.9 / 11.0 | 87 | intergenic (‑165/‑113) | yghY/yghZ | ECK2994:JW5496:b3000; predicted dienlactone hydrolase/aldo‑keto reductase |
| Reads supporting (aligned to +/- strand): ref base A (55/20); new base G (9/3); total (64/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
TTGGCGGTTAAACGCGGCATCGTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCGCTA > minE/2010481‑2010611 | ttGGCAGTTAAACGCGGCATCGTTGCACTCCTCAACTTGTGTTTTTTTAAGCAAAGCATAAGCGCGtgt < 1:1442992/69‑3 (MQ=255)ttGGCAGTTAAACGCGGCATCGTTGCACTCCTCAACTGGTGTTTTTTTAAGCAAAGCATAAGCGCGtgt < 1:1365440/69‑3 (MQ=255)ttGGCAGTTAAACGCGGCATCGTTGCACTCCTCAACTGGTGTTTTTTTAAGCAAAGCATAAGCGCGtgt < 1:1041277/69‑3 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGtt > 1:2005061/1‑60 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:1032941/1‑69 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:2624133/1‑69 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:318105/1‑69 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:1191576/1‑69 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:1113252/1‑69 (MQ=255) gTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCAc > 1:1059863/1‑69 (MQ=255) gCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGcc < 1:2771990/52‑1 (MQ=255) gCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGcc < 1:1299101/52‑1 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:285717/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:2097054/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:2890484/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:2648818/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:752831/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:2388559/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:2570338/1‑68 (MQ=255) cctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:1739870/1‑68 (MQ=255) ctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:133105/1‑67 (MQ=255) ctcAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGa > 1:736197/1‑67 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:1432153/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:144079/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:140183/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:275701/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:1108860/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:718682/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:3115640/69‑1 (MQ=255) cAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:1789615/69‑1 (MQ=255) aGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:732844/68‑1 (MQ=255) aGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:408755/68‑1 (MQ=255) aGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:1553056/68‑1 (MQ=255) ggTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTa > 1:1788851/1‑69 (MQ=255) ggTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTa > 1:2692152/1‑69 (MQ=255) ggTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTa > 1:784733/1‑69 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:861276/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:573548/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:531183/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:1163814/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:365141/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:1352844/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:2584610/1‑68 (MQ=255) tgtTTTTTTAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTAc > 1:1335876/1‑68 (MQ=255) tggttttttAAGCAAAGCATAAGCGCGTGTTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTa > 1:1465634/4‑67 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCtg > 1:3154258/1‑51 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGa < 1:1892434/68‑1 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGa < 1:3138775/68‑1 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGa < 1:2437926/68‑1 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:825251/1‑69 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:1475098/1‑69 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:1548489/1‑69 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:874497/1‑69 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:1033002/1‑69 (MQ=255) tttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGAt > 1:3030949/1‑69 (MQ=255) ttttAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGa < 1:1938478/67‑1 (MQ=255) aGCACTTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa > 1:2489245/1‑41 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAg > 1:73302/1‑38 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa > 1:2792268/1‑41 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa > 1:850417/1‑41 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa < 1:484686/41‑1 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCa > 1:464580/1‑41 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATAcgc > 1:1029738/1‑56 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATc > 1:1119464/1‑67 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1180861/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1579461/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1317193/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1240304/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1221832/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:820186/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:690163/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1150913/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:934229/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:975893/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1678633/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:2988517/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1953320/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:1972424/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:2182378/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:239248/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:3395449/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:2791431/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:2926723/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:3083244/1‑68 (MQ=255) aGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCg > 1:2984735/1‑68 (MQ=255) cACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCGCTa < 1:827271/69‑1 (MQ=255) cACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCGCTa < 1:3319694/69‑1 (MQ=255) | TTGGCGGTTAAACGCGGCATCGTTGCACTCCTCAGTTGGTGTTTTTTTAAGCAAAGCATAAGCACGTATTTTTGCCCAGTTTTTCGTCACTCTGTGAGCCAGACTACGGGATACGCGCTGGCGAATCGCTA > minE/2010481‑2010611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |