| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,012,911 | C→T | 33.6% | L164L (CTG→TTG) | yghA → | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,012,911 | 0 | C | T | 33.6% | 69.3 / 45.2 | 86 | L164L (CTG→TTG) | yghA | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (39/18); new base T (12/17); total (51/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCAC > minE/2012844‑2012972 | cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCt > 1:3341805/1‑69 (MQ=255)cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCt > 1:1300464/1‑69 (MQ=255) ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:3408614/69‑1 (MQ=255) ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:3010896/69‑1 (MQ=255) tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:1210645/68‑1 (MQ=255) tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:1631272/68‑1 (MQ=255) ggATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCt < 1:872514/68‑1 (MQ=255) gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTaa > 1:411891/1‑67 (MQ=255) gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTaa > 1:1970761/1‑67 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:3126078/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2980673/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1900478/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1875103/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1730053/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2283212/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2442040/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2568658/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2678366/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1539522/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:832544/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:600792/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:637769/1‑69 (MQ=255) aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1387217/1‑69 (MQ=255) aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATc > 1:1534279/1‑69 (MQ=255) aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATc > 1:1702671/1‑69 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:1388926/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2300724/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2840859/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2818717/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:3135757/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:3204151/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:1779652/1‑35 (MQ=255) tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2789329/1‑35 (MQ=255) aaGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaa < 1:2850905/68‑1 (MQ=255) aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:3072737/1‑69 (MQ=255) aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:2148636/1‑69 (MQ=255) aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:2215776/1‑69 (MQ=255) aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:470506/1‑69 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:2646024/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:863581/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:856740/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:694665/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:678790/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:3037357/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:487975/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:3274712/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1431761/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1062527/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1262882/69‑1 (MQ=255) cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:190913/69‑1 (MQ=255) tttGCCATTAACGTTTTCGCGTTGTTCTGGCTACCCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1851291/68‑1 (MQ=255) tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:2821821/68‑1 (MQ=255) tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1603396/68‑1 (MQ=255) tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1672807/68‑1 (MQ=255) ccATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCCAAAGGTGc < 1:2081229/64‑1 (MQ=255) ccATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg < 1:1667477/67‑1 (MQ=255) cATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:582285/68‑1 (MQ=255) cATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:373478/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:622693/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:164704/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2102677/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2234351/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2582478/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2655523/68‑1 (MQ=255) aTTAACGTTTTCGCGCTGTTCTGGCTAACACAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:3073649/67‑1 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc > 1:1357016/1‑46 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc > 1:345748/1‑46 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:2023554/1‑69 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:1168835/1‑69 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:381713/1‑69 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:62380/1‑69 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:2670414/1‑69 (MQ=255) gTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc < 1:3132614/69‑1 (MQ=255) gTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCCGCTACCGAAAGGTGCAAGTATCATcacc < 1:1339322/69‑1 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:674098/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2729118/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1396729/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1296390/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1463924/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:371091/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:3205015/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:199638/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2882929/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2345894/1‑69 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca > 1:1405135/1‑68 (MQ=255) tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca > 1:338839/1‑68 (MQ=255) gcgtTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAACGTGc < 1:1278906/50‑1 (MQ=255) | CATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCAC > minE/2012844‑2012972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |