| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,013,916 | G→A | 37.9% | L30L (CTG→TTG) | exbD ← | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,013,916 | 0 | G | A | 37.9% | 64.3 / 70.3 | 111 | L30L (CTG→TTG) | exbD | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base G (44/24); new base A (29/13); total (73/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TTCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAG > minE/2013867‑2013979 | ttCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCATAACCAACATCACGTCGATa < 1:1793564/69‑1 (MQ=255)ttCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATa > 1:26431/1‑69 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:2263678/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:1784603/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:865787/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:1550176/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:1511207/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:133603/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:2777696/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:91713/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:3028179/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:3260230/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:3270723/69‑1 (MQ=255) tCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAAAACCAACATCACGTCGATaa < 1:1059838/69‑1 (MQ=255) aTCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACgg > 1:805747/1‑69 (MQ=255) aTCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACgg > 1:1071416/1‑69 (MQ=255) cTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGc > 1:580595/1‑68 (MQ=255) cTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGc > 1:2785482/1‑68 (MQ=255) cTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGc > 1:803266/1‑68 (MQ=255) cTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGc > 1:1817670/1‑68 (MQ=255) cTACAGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACgg > 1:235644/1‑67 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:3334026/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:530625/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:1126651/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:3256049/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:2786469/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGt > 1:635538/1‑69 (MQ=255) tACCGTCGCTAACGGTGCCGCCACCAAAAAGATAATCAGCAGAACAAACATCACGTCGATAAACGGCGt > 1:3261370/1‑69 (MQ=255) gCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGtt > 1:908584/1‑68 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:544696/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:229910/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:2673117/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:1274283/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:1364664/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:927491/37‑1 (MQ=255) aCGGTGCCGCCACCATAAAGATAATCAGCAGAACCaa < 1:1607560/37‑1 (MQ=255) ggTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:440072/67‑1 (MQ=255) ggTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:528399/67‑1 (MQ=255) ggTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:1778886/67‑1 (MQ=255) ggTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:2789771/67‑1 (MQ=255) ggTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:891323/67‑1 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCTTTTCGCCGtt > 1:2615357/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCGTTTCGCCGt > 1:3107785/1‑68 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGcc > 1:3285700/1‑66 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2794823/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1786783/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:862233/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1880118/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:3115471/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:3083668/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1144559/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:3004517/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:3419967/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:542514/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2794145/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1894835/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1897024/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1987806/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:672170/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:407209/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2195766/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1287022/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:1303400/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2296897/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2355804/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGtt > 1:2580781/1‑69 (MQ=255) aaaGATAATCAGCAAAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGt > 1:1875812/1‑68 (MQ=255) aaGATAATCAGCAGGACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:1101923/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:371297/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:3396807/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:967997/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:3309034/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:1212414/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:1825146/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGatat < 1:1995047/52‑1 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCAt > 1:91342/1‑59 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:3033367/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:1048576/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:160671/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:1645702/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:660358/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:634625/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:225656/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:558039/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:2959829/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:2651942/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:2623832/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:2614786/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCAAGCATTTCGCCGTTa > 1:2412114/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTAACGTTGATATCATGCATTTCGCCGTTa > 1:2522679/1‑69 (MQ=255) aaGATAATCAGCAGAACCAACATAACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTa > 1:1947178/1‑69 (MQ=255) tAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTAtcg > 1:2870107/1‑68 (MQ=255) tAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTAtcg > 1:342891/1‑68 (MQ=255) aaTCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTAtcg > 1:1665022/1‑67 (MQ=255) aaTCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTAtcg > 1:1272791/1‑67 (MQ=255) aaTCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTAtcg > 1:2232050/1‑67 (MQ=255) cagcagAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAg < 1:790964/69‑1 (MQ=255) cagcagAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAg < 1:838533/69‑1 (MQ=255) cagcagAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAg < 1:2172198/69‑1 (MQ=255) cagcagAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAg < 1:2741221/69‑1 (MQ=255) | TTCACATCTACCGTCGCTAACGGTGCCGCCACCATAAAGATAATCAGCAGAACCAACATCACGTCGATAAACGGCGTCACGTTGATATCATGCATTTCGCCGTTATCGTCGAG > minE/2013867‑2013979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |