| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,014,019 | G→A | 48.2% | R242R (CGC→CGT) | exbB ← | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,014,019 | 0 | G | A | 48.2% | 1.1 / 47.0 | 56 | R242R (CGC→CGT) | exbB | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base G (9/20); new base A (18/9); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
TCATGCATTTCGCCGTTATCGTCGAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAGGTCAC > minE/2013954‑2014080 | tCATGCATTTCGCCGTTATCGTCGAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGt < 1:552137/69‑1 (MQ=255) gtcgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAAc > 1:322360/1‑69 (MQ=255) gtcgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAAc > 1:1322779/1‑69 (MQ=255) gtcgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAAc > 1:2319648/1‑69 (MQ=255) cgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:692533/69‑1 (MQ=255) cgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:3407145/69‑1 (MQ=255) cgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:2439881/69‑1 (MQ=255) cgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:3423653/69‑1 (MQ=255) cgAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:2249768/69‑1 (MQ=255) gAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCg < 1:1876626/68‑1 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACg > 1:1626561/1‑62 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:234267/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2236872/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2551921/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2843410/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2556039/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2850078/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:2886301/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:3162929/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1750292/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:3131535/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1694793/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1658140/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1436332/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1347871/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1341580/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:51551/1‑63 (MQ=255) aGGTTTTCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTAATTTTTGTGCGACACGa > 1:1001794/1‑63 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:311711/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:1140723/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:2679952/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:379593/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:2587099/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:2562140/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:228451/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:1189063/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:137561/68‑1 (MQ=255) ggTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGa < 1:1662003/68‑1 (MQ=255) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:243333/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:76530/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:2143550/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:2139418/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:2889443/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:2955377/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:122149/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:467569/41‑1 (MQ=25) ttCGTTAAGATGCATCGCCATCGGACATTATCCTGCACGTa < 1:1563156/41‑1 (MQ=25) tGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTg > 1:796282/1‑69 (MQ=255) tGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTg > 1:1696644/1‑69 (MQ=255) tGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTg > 1:1731907/1‑69 (MQ=255) atatTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAg < 1:1266925/69‑1 (MQ=255) atatTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAg < 1:182134/69‑1 (MQ=255) tattatCCTGCGCGTAATTTTTGTGCGACACTAACCg < 1:2591879/37‑1 (MQ=255) ttatCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAGGTc > 1:1703494/1‑69 (MQ=255) ttatCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAGGTc > 1:2079072/1‑69 (MQ=255) atCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAGGTCAc > 1:2591540/1‑69 (MQ=255) | TCATGCATTTCGCCGTTATCGTCGAGGTTTTCGTTAAGATGCATTGCCATCGGATATTATCCTGCGCGTAATTTTTGTGCGACACGAACCGGATGCGCAGCGGCGCTGGCTTCCAGATCCAGGTCAC > minE/2013954‑2014080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |