| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 162,233 | A→G | 48.7% | T316A (ACG→GCG) | cdaR → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 162,233 | 0 | A | G | 48.7% | ‑5.8 / 33.2 | 39 | T316A (ACG→GCG) | cdaR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/3); new base G (6/13); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01 | |||||||||||
TTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACAA > minE/162167‑162288 | ttACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACg > 1:1000042/1‑69 (MQ=255)ttACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACg > 1:482609/1‑69 (MQ=255) aCTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGa > 1:291111/1‑68 (MQ=255) aCTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGATGAAAACGa > 1:2063285/1‑68 (MQ=255) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2750812/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2840280/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:2138601/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:307219/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:1525687/1‑69 (MQ=39) gCAGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg > 1:699573/1‑69 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTTAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:704628/67‑1 (MQ=255) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:3390959/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2960168/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:67224/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2857349/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2832687/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:1289811/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2290912/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:197122/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:1908447/67‑1 (MQ=39) aGGCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2771442/67‑1 (MQ=39) ggCCAACGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2448465/66‑1 (MQ=39) ccAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGcg < 1:234220/61‑1 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTTAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt < 1:923367/69‑1 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt > 1:1976369/1‑69 (MQ=255) cAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCt < 1:3055377/69‑1 (MQ=255) aaCGAACTGGCGCGACCGCTGGCACGGCTGAAAGCGATGGACAATAACGGCTTGCTgcggcg < 1:2880726/62‑1 (MQ=38) gAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTggcg > 1:631234/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGcgacg > 1:1736110/1‑56 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1702766/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:522211/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:605677/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:3138386/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1611935/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:1324543/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:780534/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:306036/1‑69 (MQ=255) cTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGgcggcg > 1:95542/1‑69 (MQ=255) gcgGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACaa > 1:1418348/1‑66 (MQ=255) | TTACTCGACAGTTTGCGTGGCGACTGGCAGGCCAACGAACTGGCGCGACCGCTGGCGCGGCTGAAAACGATGGACAATAACGGCTTGCTGCGACGAACGCTGGCGGCGTGGTTTCGCCACAA > minE/162167‑162288 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |