| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,014,583 | G→A | 43.2% | R54R (CGC→CGT) | exbB ← | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,014,583 | 0 | G | A | 43.2% | 32.7 / 79.3 | 104 | R54R (CGC→CGT) | exbB | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base G (20/39); new base A (13/32); total (33/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
GATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAG > minE/2014523‑2014646 | gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGGTCGCGCTTAAGGCGACGCtt > 1:481791/1‑69 (MQ=255)gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCtt > 1:1114565/1‑69 (MQ=255)gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCtt > 1:2898717/1‑69 (MQ=255) tatCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCt < 1:285636/69‑1 (MQ=255) tatCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCt < 1:2139359/69‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:1210924/67‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:3279886/67‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:2338506/67‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:912877/67‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:3186864/67‑1 (MQ=255) ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:476836/67‑1 (MQ=255) aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:2832628/69‑1 (MQ=255) aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:467787/69‑1 (MQ=255) aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:2325246/69‑1 (MQ=255) aCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCtt > 1:804914/1‑69 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:3212675/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:410608/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2820659/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:501398/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:512382/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2556326/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:567286/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:654356/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2194435/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:465832/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2038047/1‑66 (MQ=255) cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:1815074/1‑66 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:2083515/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:3128304/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:11357/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:3266783/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:347442/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:835888/68‑1 (MQ=255) gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATCGAAGAACTCTACGCTCTTACTgaagaa < 1:1909071/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1837128/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1115103/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:122761/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:3341580/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1532221/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2811025/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2282383/68‑1 (MQ=255) ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1934645/68‑1 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:2340690/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:387281/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:199851/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:2856405/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:3263785/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:1019327/1‑43 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:1881443/69‑1 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:1763613/69‑1 (MQ=255) cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:2326508/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2161221/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:734079/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:828943/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:3227497/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:439675/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:1089154/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2776336/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:1801152/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2621651/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2579543/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:520904/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2504122/69‑1 (MQ=255) gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2372474/69‑1 (MQ=255) ttGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2330944/68‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3421818/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2102210/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3351657/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3397026/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1373434/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1379231/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2897311/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1463468/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1839333/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:619444/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1964518/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2106363/69‑1 (MQ=255) tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTAATGAAGAAGATTGCCCAg < 1:3177254/69‑1 (MQ=255) gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:663262/1‑68 (MQ=255) gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:41493/1‑68 (MQ=255) gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:1076748/1‑68 (MQ=255) gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:2897169/1‑68 (MQ=255) gctgctCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2206796/68‑1 (MQ=255) ctgctCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:398981/65‑1 (MQ=255) ctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2485577/55‑1 (MQ=255) cgcgCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2334793/60‑1 (MQ=255) cgcTTAAGACGACGCTTCTGATTGAAGAACTCCAGGCTCTTACTGAAGAAGATTGCCCAg < 1:1486925/60‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:3200928/59‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2139532/59‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2507309/59‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2719296/59‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2736329/59‑1 (MQ=255) gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:3033336/59‑1 (MQ=255) cTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2147980/58‑1 (MQ=255) ttAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTa < 1:2147349/39‑1 (MQ=255) tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:2340184/1‑68 (MQ=255) tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:2925206/1‑68 (MQ=255) tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:831126/1‑68 (MQ=255) tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:3125575/1‑68 (MQ=255) tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:1431247/1‑68 (MQ=255) | GATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAG > minE/2014523‑2014646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |