| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,019,133 | C→T | 35.6% | P340L (CCG→CTG) | yqhD → | alcohol dehydrogenase, NAD(P)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,019,133 | 0 | C | T | 35.6% | 25.7 / 20.9 | 42 | P340L (CCG→CTG) | yqhD | alcohol dehydrogenase, NAD(P)‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (16/11); new base T (2/13); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01 | |||||||||||
TCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAG > minE/2019066‑2019200 | tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCg > 1:3263226/1‑69 (MQ=255) ccGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:2951231/1‑69 (MQ=255) ccGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:466863/1‑69 (MQ=255) ccGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:446297/1‑69 (MQ=255) ccGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:1957186/1‑69 (MQ=255) ccGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:3421982/1‑69 (MQ=255) ccGATGATGAGCGTATTGACACCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGa > 1:2619560/1‑69 (MQ=255) cGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTcc < 1:1530647/69‑1 (MQ=255) cGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTcc < 1:2273515/69‑1 (MQ=255) gcaTTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:1584967/67‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTTCTGACCCACCTCTCCg < 1:2118806/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:403755/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:2921929/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:3118372/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:383833/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:2822356/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:548003/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:655346/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:968352/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:2433890/69‑1 (MQ=255) gTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:2116149/69‑1 (MQ=255) aTTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCg < 1:1224791/67‑1 (MQ=255) aTTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGAc < 1:3112798/69‑1 (MQ=255) aTTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGAc < 1:2061088/69‑1 (MQ=255) gACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACct > 1:50907/1‑59 (MQ=255) gACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGa > 1:2617603/1‑65 (MQ=255) gACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTAc > 1:2720402/1‑69 (MQ=255) gcgATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTg < 1:693346/69‑1 (MQ=255) aaCCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTc < 1:1256899/69‑1 (MQ=255) ttCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCt > 1:2312092/1‑69 (MQ=255) ttCTTTGAGCAATTAGGCGTGCTGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCt > 1:3324303/1‑69 (MQ=255) tCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCtt > 1:1110961/1‑69 (MQ=255) tCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCtt > 1:2935174/1‑69 (MQ=255) aGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAAc > 1:2138891/1‑68 (MQ=255) aGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAAc > 1:2148521/1‑68 (MQ=255) aGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAAc > 1:2260715/1‑68 (MQ=255) aGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAAc > 1:1065368/1‑68 (MQ=255) cGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTgg < 1:371482/68‑1 (MQ=255) cGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTgg < 1:1135759/68‑1 (MQ=255) tGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAag < 1:322146/69‑1 (MQ=255) tGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAag < 1:76343/69‑1 (MQ=255) gCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAag < 1:2471146/68‑1 (MQ=255) ccGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAagag < 1:1430244/69‑1 (MQ=255) ccGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAagag < 1:671737/69‑1 (MQ=255) | TCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAG > minE/2019066‑2019200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |