| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,020,144 | C→T | 47.1% | L254L (CTC→CTT) | dkgA → | 2,5‑diketo‑D‑gluconate reductase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,020,144 | 0 | C | T | 47.1% | 3.2 / 42.7 | 53 | L254L (CTC→CTT) | dkgA | 2,5‑diketo‑D‑gluconate reductase A |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base T (10/15); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTC > minE/2020082‑2020201 | acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:3404561/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:1108806/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:2805498/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:3428640/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:2609626/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:1176381/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGaa > 1:3000181/1‑69 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGa > 1:131059/1‑68 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGa > 1:2661086/1‑68 (MQ=255)acacCTTCACGCATTGTCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTTTGCGa > 1:119731/1‑68 (MQ=255) ttCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGc < 1:2822593/69‑1 (MQ=255) cACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa > 1:3142058/1‑69 (MQ=255) cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa < 1:2647293/67‑1 (MQ=255) cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa < 1:2745734/67‑1 (MQ=255) cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa < 1:457144/67‑1 (MQ=255) cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa < 1:3341284/67‑1 (MQ=255) cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCaa < 1:1148062/67‑1 (MQ=255) gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCg > 1:2637787/1‑67 (MQ=255) gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCg > 1:666373/1‑67 (MQ=255) gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCg > 1:1655110/1‑67 (MQ=255) aTGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGt < 1:481701/68‑1 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:1684719/1‑69 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:235957/1‑69 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:671372/1‑69 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:8323/1‑69 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:917950/1‑69 (MQ=255) tCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCg > 1:1118935/1‑69 (MQ=255) aTTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCg > 1:19615/1‑69 (MQ=255) aTTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCg > 1:1367732/1‑69 (MQ=255) tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGCTc > 1:707504/1‑69 (MQ=255) tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATc > 1:951930/1‑69 (MQ=255) ctcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGAc < 1:2297814/69‑1 (MQ=255) ctcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGAc < 1:2181281/69‑1 (MQ=255) ctcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGAc < 1:1207736/69‑1 (MQ=255) taaaGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:2546096/66‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1002559/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1153304/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1258330/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:71360/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1266983/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:131771/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:624584/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1896574/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:2013334/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:2386908/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:3333252/67‑1 (MQ=255) cAAAGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:2713613/67‑1 (MQ=255) cAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1566312/67‑1 (MQ=255) cAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:2311018/67‑1 (MQ=255) cAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:1046462/67‑1 (MQ=255) aaaGACGAACTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:540451/66‑1 (MQ=255) aaGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTc > 1:1407209/1‑68 (MQ=255) aCTTGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg < 1:933567/58‑1 (MQ=255) | ACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTC > minE/2020082‑2020201 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |