| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,021,534 | C→T | 31.8% | L50L (CTA→TTA) | yqhH → | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,021,534 | 0 | C | T | 31.8% | 59.9 / 32.3 | 69 | L50L (CTA→TTA) | yqhH | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (21/26); new base T (10/12); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
TCAGCTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATG > minE/2021467‑2021594 | tCAGCTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCAc < 1:3049157/68‑1 (MQ=255) aaTGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:100274/69‑1 (MQ=255) aaTGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:2334821/69‑1 (MQ=255) aaTGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:186269/69‑1 (MQ=255) aaTGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:925835/69‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:858743/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:1710720/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:1680901/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:3396853/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:2709777/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:2609416/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:2448359/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:1488843/68‑1 (MQ=255) cAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTAtgc < 1:1567118/68‑1 (MQ=255) aCATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:414580/60‑1 (MQ=255) aTTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCaa > 1:626152/1‑69 (MQ=255) aTTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCaa > 1:1590787/1‑69 (MQ=255) tAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCaaa > 1:1561758/1‑68 (MQ=255) tAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1557659/1‑69 (MQ=255) tAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1434051/1‑69 (MQ=255) tAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:131184/1‑69 (MQ=255) tAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:3226336/1‑69 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1973555/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:51673/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:3083608/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1231865/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1401757/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:1408898/1‑68 (MQ=255) aaaTGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAAt > 1:141162/1‑68 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:2810713/1‑69 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:1262236/1‑69 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:147968/1‑69 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:2400602/1‑69 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:2477939/1‑69 (MQ=255) tGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGa > 1:2529273/1‑69 (MQ=255) aaaaTCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCt < 1:624565/69‑1 (MQ=255) aaaaTCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCt < 1:1216068/69‑1 (MQ=255) aaaaTCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCt < 1:898023/69‑1 (MQ=255) cGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGcc < 1:165543/69‑1 (MQ=255) cGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGcc < 1:2901352/69‑1 (MQ=255) ggCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGcc < 1:2159042/68‑1 (MQ=255) ggCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGcc < 1:2831334/68‑1 (MQ=255) gCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCaa < 1:2155590/69‑1 (MQ=255) gCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCaa < 1:1181167/69‑1 (MQ=255) cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCaa < 1:3246526/68‑1 (MQ=255) ttGAGCAGGATATGAAAGCACTACGCCCACAAATc < 1:169124/35‑1 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCt > 1:1336397/1‑35 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:377683/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:994868/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:2774427/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:276292/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:1417110/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:1915895/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:1895361/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATAc > 1:1866695/1‑69 (MQ=255) tGAGCAGGATATGAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCAAATAc > 1:831295/1‑69 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTc > 1:2978410/1‑68 (MQ=255) tGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGGCTTGATg < 1:3017837/69‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:1020627/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:956554/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:1099055/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:1862001/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:698518/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:486310/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:478261/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:2721396/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:2754435/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:275985/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:3236548/44‑1 (MQ=255) gAAAGCATTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTa < 1:3133651/44‑1 (MQ=255) | TCAGCTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATG > minE/2021467‑2021594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |