| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,022,917 | G→A | 30.3% | H330H (CAC→CAT) | ygiQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,022,917 | 0 | G | A | 30.3% | 90.0 / 42.0 | 92 | H330H (CAC→CAT) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (35/29); new base A (9/19); total (44/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
GTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG > minE/2022857‑2022982 | gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTg < 1:590267/69‑1 (MQ=255)gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTg < 1:1155530/69‑1 (MQ=255)gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTg < 1:2561812/69‑1 (MQ=255)gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTg < 1:1929460/69‑1 (MQ=255) tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATc < 1:1606864/68‑1 (MQ=255) tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATc < 1:802565/68‑1 (MQ=255) ctctTCGGTAGGAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:1768058/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCATGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:2089286/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:2310764/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:1667268/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:30542/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:2837099/1‑69 (MQ=255) ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:1182125/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa > 1:1487783/1‑68 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:3026785/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:2393106/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:3366613/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:2314161/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:2300609/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:391442/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:1125372/1‑69 (MQ=255) tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAt > 1:824370/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:158183/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:1118474/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:1542446/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:1495510/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:2981766/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:1389767/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:791758/1‑69 (MQ=255) cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcgc > 1:2057515/1‑69 (MQ=255) aGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTCCATCaa > 1:366059/1‑60 (MQ=255) gtgTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2842892/67‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2363335/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:398853/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:866687/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2687296/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2816681/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:769479/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2840562/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:688120/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:661782/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:657783/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:337131/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:20975/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:161787/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1591747/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2086360/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1812754/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1388037/69‑1 (MQ=255) gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1969538/69‑1 (MQ=255) gggTTGATCCACACATAGCTGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1585849/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1602754/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:3134171/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1430781/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2924466/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:820201/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2392297/65‑1 (MQ=255) gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2558357/65‑1 (MQ=255) ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:794522/64‑1 (MQ=255) ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg < 1:895340/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1451155/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:2734385/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1755383/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1769760/69‑1 (MQ=255) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1716454/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1379510/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1466352/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:147416/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1563738/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:286992/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1086223/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1714260/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:2257181/1‑50 (MQ=38) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:960381/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:3299418/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2471142/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2069125/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:1903925/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:310085/1‑60 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:7740/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:1613834/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:3217155/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:453399/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3196709/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3235737/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1971212/69‑1 (MQ=255) tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:2974537/68‑1 (MQ=255) tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1950271/68‑1 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTg > 1:3242510/1‑35 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa > 1:75015/1‑58 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:1159902/1‑69 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:2361747/1‑69 (MQ=255) | GTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG > minE/2022857‑2022982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |