| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,022,941 | A→G | 38.3% | C322C (TGT→TGC) | ygiQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,022,941 | 0 | A | G | 38.3% | 35.7 / 47.4 | 73 | C322C (TGT→TGC) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/35); new base G (9/19); total (19/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 | |||||||||||
GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC > minE/2022891‑2023009 | gtgTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2842892/67‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:657783/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1812754/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2840562/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2816681/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1969538/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:161787/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:337131/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2687296/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1591747/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:398853/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:661782/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:688120/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1388037/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:769479/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2363335/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2086360/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:866687/69‑1 (MQ=255)gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:20975/69‑1 (MQ=255)gggTTGATCCACACATAGCTGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1585849/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:3134171/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2558357/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2392297/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2924466/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1602754/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1430781/65‑1 (MQ=255)gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:820201/65‑1 (MQ=255) ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:794522/64‑1 (MQ=255) ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg < 1:895340/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1769760/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1755383/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:2734385/69‑1 (MQ=255) acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1451155/69‑1 (MQ=255) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:286992/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1086223/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:2257181/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1379510/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1466352/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:147416/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1563738/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1714260/1‑50 (MQ=38) acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1716454/1‑50 (MQ=38) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2069125/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:960381/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:3299418/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:310085/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:1903925/1‑60 (MQ=255) gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2471142/1‑60 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:7740/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:453399/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:1613834/69‑1 (MQ=255) ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:3217155/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1971212/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3235737/69‑1 (MQ=255) gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3196709/69‑1 (MQ=255) tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:2974537/68‑1 (MQ=255) tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1950271/68‑1 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTg > 1:3242510/1‑35 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa > 1:75015/1‑58 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:1159902/1‑69 (MQ=255) gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:2361747/1‑69 (MQ=255) tgtTTTTGCATCAATGCGCTGGCACAGCCTGGGTTGGTTTCGTTGTGCAGAATACGCGAAGCATGGGCg < 1:1972485/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCATAATACGCGAAGCATGGGCg < 1:528051/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:2249340/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:2882664/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:1228851/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:759979/69‑1 (MQ=255) tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:737919/69‑1 (MQ=255) tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:3068084/69‑1 (MQ=255) tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:635350/69‑1 (MQ=255) tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:1574798/69‑1 (MQ=255) tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:3278566/69‑1 (MQ=255) tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:2613201/69‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:579663/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:604175/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:2064350/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1834098/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1829965/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1322065/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:259401/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1021764/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1701119/62‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2820006/69‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2419129/69‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2012668/69‑1 (MQ=255) acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:3233790/69‑1 (MQ=255) | GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC > minE/2022891‑2023009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |