| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,023,139 | A→G | 35.5% | G256G (GGT→GGC) | ygiQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,023,139 | 0 | A | G | 35.5% | 37.9 / 32.7 | 59 | G256G (GGT→GGC) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/21); new base G (13/8); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
CGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCG > minE/2023072‑2023202 | cGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAc < 1:1925539/69‑1 (MQ=255)cGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAc < 1:1513785/69‑1 (MQ=255)cGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAc < 1:437382/69‑1 (MQ=255)cGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAc < 1:551504/69‑1 (MQ=255) gTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCTGTTTGTTATACGCGCACGGAAAATCTTCAcc > 1:420874/1‑68 (MQ=255) gTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCa > 1:1383836/1‑69 (MQ=255) gTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCa > 1:2318451/1‑69 (MQ=255) gTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCa > 1:1779762/1‑69 (MQ=255) gTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCa > 1:1594830/1‑69 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:2265330/1‑58 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:189657/1‑58 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:3307570/1‑58 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:211189/1‑58 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg < 1:707192/58‑1 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:1555454/1‑58 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg < 1:1409387/58‑1 (MQ=255) tttCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg > 1:1309251/1‑58 (MQ=255) aCCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCggg < 1:1370021/48‑1 (MQ=255) ccGGTTTGTTATCCGCGCCCGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:303452/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATa > 1:2967924/1‑37 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:3137451/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:350383/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:3123423/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:662499/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:3120473/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:744122/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:3099763/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:95525/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:2696314/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:2389713/1‑61 (MQ=255) ccGGTTTGTTATCCGCGCACGGCAAATCTTCGCCATACGGATGCGGGATTGGGTCGAtttt > 1:1655375/1‑61 (MQ=255) tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:9185/69‑1 (MQ=255) tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:3007426/69‑1 (MQ=255) tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:2309829/69‑1 (MQ=255) tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:483896/69‑1 (MQ=255) tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:185998/69‑1 (MQ=255) gTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:238668/68‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:2764204/69‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:735356/69‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:2517064/69‑1 (MQ=255) cGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1790401/69‑1 (MQ=255) cGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1145868/69‑1 (MQ=255) ggCATATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1441901/68‑1 (MQ=255) cAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:1074611/53‑1 (MQ=255) aaaTCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:1712904/56‑1 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:3282424/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1714302/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1984730/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:488292/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:2847823/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1591362/69‑3 (MQ=255) aTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:2729898/68‑3 (MQ=255) aTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:815131/68‑3 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGa > 1:3368074/1‑51 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:742739/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:2849726/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:156964/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:580422/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:2205513/1‑69 (MQ=255) | CGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCG > minE/2023072‑2023202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |