| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,023,172 | A→T | 25.8% | P245P (CCT→CCA) | ygiQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,023,172 | 0 | A | T | 25.8% | 90.4 / 32.0 | 62 | P245P (CCT→CCA) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (19/27); new base T (6/10); total (25/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATAA > minE/2023114‑2023233 | tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:9185/69‑1 (MQ=255)tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:483896/69‑1 (MQ=255)tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:3007426/69‑1 (MQ=255)tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:2309829/69‑1 (MQ=255)tGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:185998/69‑1 (MQ=255) gTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:238668/68‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:735356/69‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:2764204/69‑1 (MQ=255) aTCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:2517064/69‑1 (MQ=255) cGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1145868/69‑1 (MQ=255) cGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1790401/69‑1 (MQ=255) ggCATATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATc < 1:1441901/68‑1 (MQ=255) cAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa < 1:1074611/53‑1 (MQ=255) aaaTCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACg < 1:1712904/56‑1 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:488292/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1591362/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1714302/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:2847823/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:3282424/69‑3 (MQ=255) aaTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1984730/69‑3 (MQ=255) aTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:2729898/68‑3 (MQ=255) aTCTTCGCCATACGGATGCGGGATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:815131/68‑3 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGa > 1:3368074/1‑51 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:580422/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:2205513/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:2849726/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:742739/1‑69 (MQ=255) tCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCAcgccg > 1:156964/1‑69 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATc > 1:2553086/1‑36 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATc > 1:1549629/1‑36 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATc > 1:2034274/1‑36 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATc > 1:1180765/1‑36 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCaa > 1:27136/1‑38 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGAc < 1:2252699/41‑1 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGa > 1:1876662/1‑48 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTccagcca > 1:1335979/1‑67 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTccagcca > 1:1719977/1‑67 (MQ=255) cGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTccagcca > 1:1558430/1‑67 (MQ=255) gATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCctc > 1:2192304/1‑69 (MQ=255) gATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCctc > 1:3406079/1‑69 (MQ=255) gATTGGGTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1513253/47‑3 (MQ=38) ggTCGATTTTCCCTGGGGTATCAAGACGGGTGGAATCCAcac < 1:1426810/42‑3 (MQ=37) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:2635725/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:3257487/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:2887622/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:2883613/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:1359432/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:1770462/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:2252828/69‑1 (MQ=255) gATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGAt < 1:2047946/69‑1 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGac > 1:2752343/1‑68 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGAt > 1:1669257/1‑69 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGAt > 1:813357/1‑69 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGAt > 1:1389721/1‑69 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGAt > 1:649188/1‑69 (MQ=255) gATTTTCCCTGGGGTATCAAGACGGGTGGAATCCACACCGCTCCAGCCAGGCAACGCCTCTTTTACGAt > 1:798152/1‑69 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATaa > 1:1258582/1‑69 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATaa > 1:1223836/1‑69 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATa < 1:178118/68‑1 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATa < 1:2418023/68‑1 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATa < 1:3300664/68‑1 (MQ=255) tttttCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATa < 1:920195/68‑1 (MQ=255) | TGTTATCCGCGCACGGCAAATCTTCACCATACGGATGCGGGATTGGGTCGATTTTTCCAGGGGTATCAAGACGGGTGGAATCCACGCCGCTCCAGCCAGGCAGCGCCTCTTTCACGATAA > minE/2023114‑2023233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |