| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,026,074 | A→G | 18.2% | R56R (CGT→CGC) | plsC ← | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,026,074 | 0 | A | G | 18.2% | 131.6 / 18.8 | 87 | R56R (CGT→CGC) | plsC | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (44/27); new base G (14/2); total (58/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
TCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGT > minE/2026009‑2026133 | tCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACgg > 1:1831078/1‑69 (MQ=255)tCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACgg > 1:1784877/1‑69 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTc > 1:1765111/1‑68 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:922403/1‑69 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2492909/1‑69 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2473377/1‑69 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2290745/1‑69 (MQ=255) ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:3171847/1‑69 (MQ=255) tgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa < 1:697058/68‑1 (MQ=255) tgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa < 1:2863510/68‑1 (MQ=255) cTGGTGGTTAGCAATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGCAGGTTTGCGGCACTCCACtt < 1:2849073/68‑1 (MQ=255) tggtggTTAGCAATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGCAGGTTTGCGGCACTCCACtt < 1:1086279/67‑1 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCa > 1:2435182/1‑68 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:258952/1‑69 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:89299/1‑69 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:2263169/1‑69 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:3216169/1‑69 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:2891435/1‑69 (MQ=255) gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg > 1:3139856/1‑69 (MQ=255) gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc > 1:70398/1‑67 (MQ=255) gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc > 1:2184120/1‑67 (MQ=255) gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc > 1:207070/1‑67 (MQ=255) gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc > 1:1434321/1‑67 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTc > 1:2923946/1‑42 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2033353/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2600205/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:71915/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2403317/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:2384089/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:3264506/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:1400889/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:219496/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:1724531/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:420788/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:650791/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa > 1:1458153/1‑43 (MQ=255) agCATTGCCGTAGCTTTCAGCGTCAGTAGGTTTACGGCACTc > 1:2927818/1‑42 (MQ=255) aTTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACg < 1:3248247/69‑1 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:589093/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:3328055/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:3347250/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:2524675/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:2696406/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:34105/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:1597981/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:1616941/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:1131464/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:1329084/1‑68 (MQ=255) gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc > 1:2109869/1‑68 (MQ=255) gTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAATCAGCGGCGCAAGACGGCCaaa < 1:1610365/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGGCAAACa < 1:1361266/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:211478/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:3352703/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:1336899/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:1725450/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:3056169/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:992567/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:948585/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:250744/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:2631474/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:2705186/69‑1 (MQ=255) aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:1188259/69‑1 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAAct < 1:9166/68‑2 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:2168344/68‑1 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:927853/68‑1 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:1807576/68‑1 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:2857404/68‑1 (MQ=255) gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa < 1:2232495/68‑1 (MQ=255) tttCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACata < 1:713901/68‑1 (MQ=255) tttCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACata < 1:1475626/68‑1 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCaaa > 1:2529612/4‑66 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg > 1:881776/4‑68 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg > 1:1634744/4‑68 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg > 1:2651517/4‑68 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:34158/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:1627967/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:1675382/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:1676702/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:853215/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:2507776/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:2675137/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:2514644/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:1073609/4‑69 (MQ=255) tgcaGGTTTGCGGCACTCCACTTTAAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt > 1:587672/4‑69 (MQ=255) tGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt < 1:2644831/69‑1 (MQ=255) tGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt < 1:2588707/69‑1 (MQ=255) tAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt < 1:162485/67‑1 (MQ=255) | TCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGT > minE/2026009‑2026133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |