| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,028,605 | G→C | 22.3% | R43R (CGC→CGG) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,028,605 | 0 | G | C | 22.3% | 227.8 / 60.4 | 130 | R43R (CGC→CGG) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (33/68); new base C (15/14); total (48/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
ACGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATG > minE/2028539‑2028668 | aCGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcggcg > 1:856376/1‑69 (MQ=255)aCGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcggcg > 1:531931/1‑69 (MQ=255)aCGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcggcg > 1:495909/1‑69 (MQ=255)aCGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcggcg > 1:2857346/1‑69 (MQ=255) ggCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTg < 1:3205075/69‑1 (MQ=255) gATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACa < 1:1213098/69‑1 (MQ=255) tttttAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcggc > 1:1378000/1‑58 (MQ=255) ggCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCaa < 1:971207/68‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:1878415/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:3221231/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:2519928/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACg > 1:2544898/1‑68 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACg > 1:2633868/1‑68 (MQ=255) cAGGCCCAGTTCAGACATCGCATACA‑AATGCGGCGCTGAACAGGTTTCAGACCAt < 1:2468136/55‑1 (MQ=255) ggCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:2313620/68‑1 (MQ=255) gccccAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:1880560/66‑1 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:1355649/1‑68 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:2394232/1‑68 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:889724/1‑68 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:3375931/1‑68 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:1695098/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2132726/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2104881/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2397584/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:1840560/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:809068/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:1086444/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2301913/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2893610/44‑1 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATaa > 1:1120716/1‑46 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAAc > 1:2388128/1‑48 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:1221571/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2318503/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2303688/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2303769/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2430533/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:3066465/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:876834/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATc > 1:1092388/1‑69 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATc > 1:885774/1‑69 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACAATCACCAATAAACGGc > 1:818066/1‑51 (MQ=255) tccaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:338636/67‑1 (MQ=255) tccaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:3379969/67‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCGACGCACGGTCCATGATCACGTa < 1:706171/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1105164/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2881161/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:1252117/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2981076/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1504761/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:645493/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:652316/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1555527/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2468701/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:3260987/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2417995/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:841762/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1026220/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2237093/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3429433/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:403197/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1146290/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3034255/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3312190/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1247753/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1007228/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:45657/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1279349/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2057811/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2212083/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2245144/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:543235/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1504819/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2612034/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1533014/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1086792/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:842295/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1775505/69‑1 (MQ=255) caATGCGCCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3095526/69‑1 (MQ=255) aaTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:996045/68‑1 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1016815/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1100415/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1163548/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2698230/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1182068/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:55981/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2608177/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2575203/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2169903/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1760084/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1584574/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTc > 1:3199105/1‑54 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACAAATAAACGGCAACGCACGGTcc > 1:2376840/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCA‑CAATAAACGGCAACGCACGGTcc > 1:3360050/1‑54 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2499361/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:1359734/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2041247/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:263808/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2741260/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2855394/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:3282126/69‑1 (MQ=255) | ACGGGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATG > minE/2028539‑2028668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |