| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,028,614 | A→G | 21.6% | P40P (CCT→CCC) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,028,614 | 0 | A | G | 21.6% | 182.5 / 39.1 | 129 | P40P (CCT→CCC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base A (34/67); new base G (15/13); total (49/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
GATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAG > minE/2028546‑2028674 | gATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACa < 1:1343950/69‑1 (MQ=255) tGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCaa < 1:2417903/69‑1 (MQ=255) ggCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCaa < 1:971207/68‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:3376127/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:3221231/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCAt < 1:2519928/56‑1 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACg > 1:3308551/1‑68 (MQ=255) cAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACg > 1:1126273/1‑68 (MQ=255) cAGGCCCAGTTCAGACATCGCATACA‑AATGCGGCGCTGAACAGGTTTCAGACCAt < 1:2468136/55‑1 (MQ=255) ggCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:872389/68‑1 (MQ=255) ggCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:839278/68‑1 (MQ=255) ggCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:1231912/68‑1 (MQ=255) ggCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:2313620/68‑1 (MQ=255) gccccAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc < 1:1880560/66‑1 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:2394232/1‑68 (MQ=255) cAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACgg > 1:1355649/1‑68 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2132726/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2104881/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2397584/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2893610/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:1840560/44‑1 (MQ=255) aTCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCAcc < 1:2301913/44‑1 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATaa > 1:1120716/1‑46 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2430533/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2433741/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:90864/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:3066465/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:1822418/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2387741/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:876834/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2318503/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2582595/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2106320/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:1221571/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2303769/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGc > 1:2303688/1‑51 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATc > 1:1092388/1‑69 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATc > 1:885774/1‑69 (MQ=255) gCATACACAATGCGGCGCTGAACAGGTTTCAGACAATCACCAATAAACGGc > 1:818066/1‑51 (MQ=255) tccaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:338636/67‑1 (MQ=255) tccaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:3379969/67‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCGACGCACGGTCCATGATCACGTa < 1:706171/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGa > 1:698520/1‑62 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2517076/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1555527/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:652316/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2634602/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:2981076/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:3260987/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1026220/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1105164/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:841762/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa > 1:1252117/1‑69 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1504761/69‑1 (MQ=255) cacaATGCGGCGCTGAACAGGTTTCAAACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTa < 1:1095110/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1007228/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3312190/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1625323/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3031716/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:403197/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:45657/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1954308/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1247753/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2212083/69‑1 (MQ=255) caATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2237093/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2778237/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2859509/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1086792/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2612034/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:621032/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1775505/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:543235/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3139947/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1083781/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1504819/69‑1 (MQ=255) caATGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:1533014/69‑1 (MQ=255) caATGCGCCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:3095526/69‑1 (MQ=255) aaTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:996045/68‑1 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1016815/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1163548/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1182068/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2698230/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:55981/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1584574/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2608177/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:2575203/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTcc > 1:1760084/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCACAAATAAACGGCAACGCACGGTcc > 1:2376840/1‑55 (MQ=255) aaTGCGCCGCTGAACGGGTTTCAGACCATCA‑CAATAAACGGCAACGCACGGTcc > 1:3360050/1‑54 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2499361/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:1189848/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:1359734/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2041247/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:263808/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:2855394/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:365165/69‑1 (MQ=255) aTGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATg < 1:3223824/69‑1 (MQ=255) aTGCGCCGCTGAACGGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACa < 1:2227734/67‑1 (MQ=255) cgcTGAACAGGATTCAGACCATCACCAATAAACGGc < 1:3230025/36‑1 (MQ=255) gcTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGagtag > 1:606889/1‑68 (MQ=255) | GATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAG > minE/2028546‑2028674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |