| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,029,444 | C→A | 31.6% | A467A (GCG→GCT) | parE ← | DNA topoisomerase IV, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,029,444 | 0 | C | A | 31.6% | 181.3 / 89.1 | 142 | A467A (GCG→GCT) | parE | DNA topoisomerase IV, subunit B |
| Reads supporting (aligned to +/- strand): ref base C (40/57); new base A (27/18); total (67/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
CGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGG > minE/2029378‑2029505 | cgcgAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGc > 1:544189/1‑69 (MQ=255) gATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGa < 1:1709549/69‑1 (MQ=255) gATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGa < 1:3022247/69‑1 (MQ=255) tACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGaaa > 1:735982/1‑69 (MQ=255) tACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGaa > 1:290254/1‑68 (MQ=255) tACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGaa > 1:3390660/1‑68 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTGAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:2153203/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:1001613/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:1614745/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:2854020/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:1304065/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:3135471/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:80093/68‑1 (MQ=255) cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACt < 1:808266/68‑1 (MQ=255) tAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTcc > 1:3018901/1‑69 (MQ=255) tAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGAACTTcc > 1:1866315/1‑69 (MQ=255) tAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACC‑AAATATCGTGCACTTCCt > 1:1217184/1‑69 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTcc > 1:1324480/1‑67 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:1286627/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:34069/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:406582/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:1085008/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:1440998/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:1676393/1‑68 (MQ=255) aCGAAGCTGGCTCAGATCGTCGCTGACAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCt > 1:1001117/1‑68 (MQ=255) gCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTTCACt < 1:2584349/59‑1 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCAc > 1:1573195/1‑47 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:3191058/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:2610615/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:2696254/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:2485038/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:493018/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:270823/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:43540/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:2877564/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1558204/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1989413/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:68396/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1951207/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1847023/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1793863/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:1791456/1‑48 (MQ=255) tcgtcgCTGTCAGGATCGATACCGATAGCTACCGAAATATCGTGCACt > 1:3027525/1‑48 (MQ=255) tcgtcgCAGTCAGGATCGATACCGATAGCTACAGaa > 1:2903015/1‑36 (MQ=25) cgtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTg < 1:2067471/43‑1 (MQ=255) cgtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTg < 1:1416646/43‑1 (MQ=255) gCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTtcgtc > 1:1885419/1‑66 (MQ=255) gCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTtcgtc > 1:1299527/1‑66 (MQ=255) gCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGa < 1:261513/69‑1 (MQ=255) gTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAaga < 1:372631/69‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:3203765/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:318573/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:929282/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:2462865/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:2354541/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:566892/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:602564/67‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:1542099/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:1030332/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:2134435/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:371981/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:2230381/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:531284/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:2805502/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:483732/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAgaga < 1:2591248/68‑1 (MQ=255) aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCAGAAgaga < 1:1283408/68‑1 (MQ=255) ggATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAagag < 1:339679/66‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:3347056/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1120414/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1611086/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1325989/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:2977574/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1782881/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:2915496/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1988643/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:2477949/69‑1 (MQ=255) cGATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:1433346/69‑1 (MQ=255) gATACCGATAGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc < 1:651895/68‑1 (MQ=255) tACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg < 1:920118/69‑1 (MQ=255) tACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg < 1:3289096/69‑1 (MQ=255) tACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg < 1:3365564/69‑1 (MQ=255) tACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg < 1:1082304/69‑1 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:2188199/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:2502967/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:614579/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:653458/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:1716189/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:1691736/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:2335040/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc < 1:1136458/39‑1 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc < 1:2343246/39‑1 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:3161238/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:2877867/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:2952991/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:1413373/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:3235471/1‑39 (MQ=255) aCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGc > 1:3150492/1‑39 (MQ=255) cGCTACCGAAATATCGTTCACTTCCTGCGAAGCCAGc < 1:1250870/37‑1 (MQ=255) cGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGc < 1:1216654/37‑1 (MQ=255) | CGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGG > minE/2029378‑2029505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |