| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,031,586 | G→A | 43.0% | S232S (TCC→TCT) | cpdA ← | cyclic 3',5'‑adenosine monophosphate phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,031,586 | 0 | G | A | 43.0% | 24.8 / 58.2 | 79 | S232S (TCC→TCT) | cpdA | cyclic 3',5'‑adenosine monophosphate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (15/30); new base A (14/20); total (29/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
CATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCA > minE/2031534‑2031648 | cATGTAACTCTAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:131271/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:1042988/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2861100/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2533831/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2387141/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2052716/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2050287/68‑1 (MQ=255)cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:1066567/68‑1 (MQ=255) aTGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTa < 1:2334101/67‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCTCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:1391765/69‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:1325663/69‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:1493668/69‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:391528/69‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:854737/69‑1 (MQ=255) tGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 1:1921702/69‑1 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:2556940/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:241112/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:2325333/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:2632713/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:3376192/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:707393/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:2049450/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:1849149/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:1017379/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:3155201/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:1642368/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:2775761/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:3105886/1‑69 (MQ=255) tAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTg > 1:1542318/1‑69 (MQ=255) cGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGAc > 1:2626624/1‑69 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2655059/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:941262/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:3186463/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2568227/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:305473/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2643034/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2717331/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2738161/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2911755/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:3328728/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:3349640/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:1169891/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2226240/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:349343/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2210564/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2173316/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:2055198/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:1889881/68‑1 (MQ=255) ccAGCCGGGCGCGATGGTGTCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:1490288/68‑1 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:2348941/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:3187425/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:1350919/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:1595195/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:3029936/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:170311/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:2868406/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:869512/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:2855675/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:28197/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:1817335/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:1856776/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAagcg > 1:273016/4‑68 (MQ=255) gtgtCCAGCGTAAAGTTAGAACAGTGCGGCTTAAACTACACACAGGTCGACGGCGTTGCCAGCAagcg > 1:286766/4‑68 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:32399/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:477445/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:98181/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:2830018/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:2773055/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:2768861/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:1171559/68‑1 (MQ=255) aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAggcggc < 1:1777807/68‑1 (MQ=255) cAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGAc < 1:1186151/68‑1 (MQ=255) cAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGAc < 1:1757410/68‑1 (MQ=255) cAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGAc < 1:1194504/68‑1 (MQ=255) aGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGAc < 1:2210872/67‑1 (MQ=255) cGTAAAGTTAGAACAGTGCGGCTTAAACTGCACACAGGTCGACg < 1:281481/44‑1 (MQ=255) aaaGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCa < 1:3160199/69‑1 (MQ=255) aaaGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCa < 1:2052669/69‑1 (MQ=255) aaGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCa < 1:1207586/68‑1 (MQ=255) | CATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCA > minE/2031534‑2031648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |