| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,039,441 | A→G | 27.1% | I158I (ATT→ATC) | ribB ← | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,039,441 | 0 | A | G | 27.1% | 111.6 / 42.1 | 103 | I158I (ATT→ATC) | ribB | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (36/39); new base G (18/10); total (54/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAA > minE/2039376‑2039505 | ccatcgtCATTAGTCAGCTCACTCAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:3165614/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:1927958/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:1044018/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:1105718/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:1649366/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:2746702/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:2789067/1‑69 (MQ=255)ccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATa > 1:3072632/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:3062168/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:2362080/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1698005/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:26208/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1462779/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:423965/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1059016/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:6027/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:517905/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:43138/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1264643/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1196925/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:419976/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:404128/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGACAGCGTCATCAGATCAATAGTTGc > 1:2527239/1‑69 (MQ=255) gtCATTAGTCAGCTCACACAGCACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:136824/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2629039/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2483622/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg > 1:2397273/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:294180/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg > 1:942460/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2006457/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:1526684/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:1088894/69‑1 (MQ=255) aGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2966667/68‑1 (MQ=255) aGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGt > 1:720559/1‑69 (MQ=255) cAGCTCACACAGTACACCAGCCTGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTAtt < 1:1746555/69‑2 (MQ=255) cAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATg < 1:1262232/69‑1 (MQ=255) cAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATg < 1:1407863/69‑1 (MQ=255) cacacaGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGc < 1:2559759/69‑1 (MQ=255) acacaGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGc < 1:2849459/68‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:342002/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1433078/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2914886/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1083583/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:37254/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1244698/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1178563/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:795059/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2262535/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2263251/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1552889/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2155154/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2073492/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:196988/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1735919/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2265927/69‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATTAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:1423661/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:1909524/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:3089878/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:2357450/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:1422315/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:805594/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:2556275/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:815406/68‑1 (MQ=255) tttAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:262626/41‑1 (MQ=255) ttAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGc < 1:65531/49‑1 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:2033998/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:427933/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:40483/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:2499151/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:1540101/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:1504813/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:1417289/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcg > 1:2188271/1‑35 (MQ=255) gATCGATAGTCGCTTCAGTATGCCCGCCACGCGTCAGTACACCACCTGCCTgcgca > 1:644066/1‑55 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGa > 1:3205240/1‑59 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGt > 1:512270/1‑62 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTg > 1:2357946/1‑63 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3166001/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2482290/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:697736/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:546245/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:522846/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:470323/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3123109/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3217824/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3051977/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2114579/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1275433/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1259955/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAACTGGGaaaa > 1:3054708/1‑69 (MQ=255) gATCGAAAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:435884/1‑69 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1084050/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3327770/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2628769/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2176274/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:44283/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3015684/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1112096/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:501995/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:466737/68‑1 (MQ=255) | CCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAA > minE/2039376‑2039505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |