| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,039,447 | T→C | 39.8% | A156A (GCA→GCG) | ribB ← | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,039,447 | 0 | T | C | 39.8% | 48.5 / 80.2 | 113 | A156A (GCA→GCG) | ribB | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (29/39); new base C (35/10); total (64/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCC > minE/2039381‑2039512 | gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1462779/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:6027/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:517905/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:43138/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:423965/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:419976/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:3062168/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1698005/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:26208/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:2362080/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1264643/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:1059016/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGACAGCGTCATCAGATCAATAGTTGc > 1:2527239/1‑69 (MQ=255)gtCATTAGTCAGCTCACACAGCACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGc > 1:136824/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg > 1:2397273/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2483622/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2006457/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2629039/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:1526684/69‑1 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg > 1:942460/1‑69 (MQ=255) tAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:1088894/69‑1 (MQ=255) aGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:2966667/68‑1 (MQ=255) aGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGt > 1:3075702/1‑69 (MQ=255) aGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGt > 1:720559/1‑69 (MQ=255) cAGCTCACACAGTACACCAGCCTGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTAtt < 1:1746555/69‑2 (MQ=255) cAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATg < 1:1262232/69‑1 (MQ=255) cAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATg < 1:1407863/69‑1 (MQ=255) acacaGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGc < 1:2849459/68‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2073492/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2265927/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2263251/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2262535/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1433078/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:342002/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:37254/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2155154/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1244698/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1178563/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:2914886/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:196988/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:795059/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1083583/69‑1 (MQ=255) acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTc < 1:1735919/69‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATTAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:1423661/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:2357450/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:2556275/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:3089878/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:1422315/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:805594/68‑1 (MQ=255) ccAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAg < 1:815406/68‑1 (MQ=255) tttAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAg < 1:262626/41‑1 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:40483/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:427933/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:1504813/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:2499151/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:1540101/1‑36 (MQ=255) atcaGATCAATAGTTGCTTCAGTATGACCGCCAcgc > 1:2033998/1‑36 (MQ=255) gATCGATAGTCGCTTCAGTATGCCCGCCACGCGTCAGTACACCACCTGCCTgcgca > 1:644066/1‑55 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGa > 1:3205240/1‑59 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGt > 1:512270/1‑62 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2114579/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:697736/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:546245/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:522846/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1259955/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3217824/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3123109/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2482290/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3051977/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1275433/1‑69 (MQ=255) gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAACTGGGaaaa > 1:3054708/1‑69 (MQ=255) gATCGAAAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:435884/1‑69 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:44283/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:466737/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1112096/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2037603/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3015684/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2176274/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:501995/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2628769/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3327770/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1084050/68‑1 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCAcctgcc > 1:118063/3‑47 (MQ=39) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACg > 1:284666/3‑68 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1686953/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1646717/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:657071/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1469614/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2277825/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1823740/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1073749/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:531306/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1326178/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1882218/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2336842/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2214028/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3361660/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3230613/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:224411/3‑69 (MQ=255) gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGcc > 1:2836692/1‑68 (MQ=255) | GTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCC > minE/2039381‑2039512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |