| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,039,489 | A→C | 60.6% | A142A (GCT→GCG) | ribB ← | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,039,489 | 0 | A | C | 60.6% | 71.2 / 71.0 | 91 | A142A (GCT→GCG) | ribB | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (27/9); new base C (33/22); total (60/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGC > minE/2039437‑2039554 | gATCGATAGTCGCTTCAGTATGCCCGCCACGCGTCAGTACACCACCTGCCTgcgca > 1:644066/1‑55 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGa > 1:3205240/1‑59 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGt > 1:512270/1‑62 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTg > 1:2357946/1‑63 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3051977/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:470323/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2114579/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:522846/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:546245/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3217824/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2482290/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1275433/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:697736/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3166001/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1259955/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3123109/1‑69 (MQ=255)gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAACTGGGaaaa > 1:3054708/1‑69 (MQ=255)gATCGAAAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:435884/1‑69 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2628769/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3327770/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2176274/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3015684/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2037603/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1084050/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:44283/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:466737/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1112096/68‑1 (MQ=255) aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:501995/68‑1 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACg > 1:284666/3‑68 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:657071/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1073749/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1469614/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3230613/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:531306/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1882218/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1326178/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3361660/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2336842/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2277825/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:224411/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2214028/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1646717/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1686953/3‑69 (MQ=255) cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1823740/3‑69 (MQ=255) gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGcc > 1:2836692/1‑68 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGATAACGTGGCCAGGACGa > 1:73569/1‑68 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGa > 1:2796655/1‑68 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGTCGATTCAg < 1:3332930/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1633184/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2296216/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2212691/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2128639/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2838746/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2029147/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1247840/69‑1 (MQ=255) aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1946475/69‑1 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:695006/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:757953/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2427689/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:1620142/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2423645/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2398858/1‑51 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:2993775/1‑69 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1630962/1‑69 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1731172/1‑69 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:3354820/1‑69 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGAGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1927757/1‑69 (MQ=255) aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt > 1:1867957/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCTAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:2948814/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1172480/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:445393/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:3258596/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1364981/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1368858/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:2337069/1‑68 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCAAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1692217/1‑68 (MQ=255) ctgCGCACGAAGTGGGAAAACGTGGCCCGTACGATTCAg < 1:575920/39‑1 (MQ=21) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCat < 1:85148/39‑2 (MQ=25) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:528875/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1270260/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1272388/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1282311/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1481927/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:2646450/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:2427070/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1853939/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1911636/39‑1 (MQ=37) ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:3383763/39‑1 (MQ=37) ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:2694111/1‑69 (MQ=255) ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:73455/1‑69 (MQ=255) ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:2404874/1‑69 (MQ=255) ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:38024/1‑69 (MQ=255) | GATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGC > minE/2039437‑2039554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |